Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
School of Pharmacy, Zunyi Medical University, Zunyi, 563000, China.
Mikrochim Acta. 2021 Apr 20;188(5):166. doi: 10.1007/s00604-021-04829-7.
A sensitive photoelectrochemical (PEC) sensor based on hexagonal carbon nitride tubes (HCNT) as photoactive material was prepared for the detection of human epidermal growth factor receptor 2 (HER2). Magnetic FeO nanospheres (MNs) modified with anti-HER2 antibodies were employed for highly efficient capture of HER2 from serum sample, and CoO nanoparticles (CoO NPs) modified with ascorbic acid oxidase (AAO) as well as HER2 aptamer were used for signal amplification. When the aptamer-CoO-AAO probe was captured onto the electrode surface through the specific binding of the aptamer with HER2, the photocurrent intensity decreased. This was because CoO NPs competed with HCNT for consumption of the excitation energy. As a consequence AAO catalyzed the oxidation of the electron donor (AA), and the aptamer-CoO-AAO probe increased the steric hindrance at the electrode surface, leading to significant photocurrent intensity decrease, thus realizing multiple signal amplification. Based on this signal amplification strategy, at 0 V (vs Ag/AgCl), the PEC sensor shows a wide linear response ranging from 1 pg mL to 1 ng mL with a low detection limit of 0.026 pg mL for HER2. Importantly, the prepared PEC sensor was applied for detection of HER2 in human serum samples with recoveries between 98.8 and 101%. Sensitive photoelectrochemical sensor based on CoO nanoparticles modified with ascorbic acid oxidase for signal amplification is reported.
基于六方氮化碳管（HCNT）作为光活性材料的灵敏光电化学（PEC）传感器，用于检测人表皮生长因子受体 2（HER2）。磁性 FeO 纳米球（MNs）修饰有抗 HER2 抗体，用于从血清样品中高效捕获 HER2，以及 CoO 纳米颗粒（CoO NPs）修饰有抗坏血酸氧化酶（AAO）和 HER2 适体，用于信号放大。当适体-CoO-AAO 探针通过适体与 HER2 的特异性结合被捕获到电极表面时，光电流强度降低。这是因为 CoO NPs 与 HCNT 竞争消耗激发能量。结果，AAO 催化电子供体（AA）的氧化，适体-CoO-AAO 探针增加了电极表面的空间位阻，导致光电流强度显著降低，从而实现了多重信号放大。基于这种信号放大策略，在 0 V（相对于 Ag/AgCl）下，PEC 传感器对 HER2 的线性响应范围从 1 pg mL 到 1 ng mL，检测限低至 0.026 pg mL。重要的是，所制备的 PEC 传感器用于检测人血清样品中的 HER2，回收率在 98.8%至 101%之间。报道了一种基于修饰有抗坏血酸氧化酶的 CoO 纳米颗粒的灵敏光电化学传感器，用于信号放大。
