School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332.
School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). doi: 10.1073/pnas.2022874118.
Itch arising from glabrous skin (palms and soles) has attracted limited attention within the field due to the lack of methodology. This is despite glabrous itch arising from many medical conditions such as plantar and palmar psoriasis, dyshidrosis, and cholestasis. Therefore, we developed a mouse glabrous skin behavioral assay to investigate the contribution of three previously identified pruriceptive neurons in glabrous skin itch. Our results show that MrgprA3 and MrgprD neurons, although key mediators for hairy skin itch, do not play important roles in glabrous skin itch, demonstrating a mechanistic difference in itch sensation between hairy and glabrous skin. We found that MrgprC11 neurons are the major mediators for glabrous skin itch. Activation of MrgprC11 neurons induced glabrous skin itch, while ablation of MrgprC11 neurons reduced both acute and chronic glabrous skin itch. Our study provides insights into the mechanisms of itch and opens up new avenues for future glabrous skin itch research.
无毛皮肤（手掌和脚底）引起的瘙痒由于缺乏方法而在该领域受到的关注有限。尽管许多医学病症都会引起无毛瘙痒，例如足底和手掌银屑病、汗疱疹和胆汁淤积，但这种情况还是存在。因此，我们开发了一种小鼠无毛皮肤行为检测方法，以研究先前鉴定的三种瘙痒神经元在无毛皮肤瘙痒中的作用。我们的结果表明，MrgprA3 和 MrgprD 神经元虽然是毛发皮肤瘙痒的主要介质，但在无毛皮肤瘙痒中不起重要作用，这表明毛发皮肤和无毛皮肤的瘙痒感觉在机制上存在差异。我们发现 MrgprC11 神经元是无毛皮肤瘙痒的主要介质。MrgprC11 神经元的激活会引起无毛皮肤瘙痒，而 MrgprC11 神经元的消融会减少急性和慢性无毛皮肤瘙痒。我们的研究为瘙痒机制提供了新的见解，并为未来的无毛皮肤瘙痒研究开辟了新的途径。
