增强酿酒酵母中的分解代谢物调节基因，以利用红海草（Gloiopeltis furcata）水解物提高乙醇产量。

Enhancement of catabolite regulatory genes in Saccharomyces cerevisiae to increase ethanol production using hydrolysate from red seaweed Gloiopeltis furcata.

机构信息

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea.

Department of Chemistry, Umeå University, SE-90187, Umeå, Sweden.

出版信息

J Biotechnol. 2021 Jun 10;333:1-9. doi: 10.1016/j.jbiotec.2021.04.004. Epub 2021 Apr 18.

DOI:10.1016/j.jbiotec.2021.04.004

PMID:33878391

Abstract

Glucose and galactose are monosaccharides obtained from Gloiopeltis furcata (Red algae). A total monosaccharide yield of 62.3 g/L was obtained from G. furcata using thermal acid hydrolysis and enzymatic saccharification. Activated carbon was used to eliminate hydroxymethylfurfural (HMF) from the hydrolysate. Previously obtained monosaccharides are used for ethanol production by Saccharomyces cerevisiae. S. cerevisiae consumes glucose first, then galactose. The methods for reducing fermentation time and increasing the ethanol yield coefficient using the simultaneous consumption of glucose and galactose have been evaluated. Gal3p and Gal80p of S. cerevisiae act as signal transducers that govern the galactose inducer Gal4p mediated transcriptional activation of the Gal gene family. Gal80p binds to Gal4p for transcription deactivation. Therefore, Gal80p was deleted for Gal4p expression without interruption.

摘要

葡萄糖和半乳糖是从鹧鸪菜（红藻）中获得的单糖。通过热酸水解和酶解，从鹧鸪菜中获得了 62.3g/L 的总单糖产量。活性炭用于从水解物中去除羟甲基糠醛（HMF）。先前获得的单糖用于酿酒酵母生产乙醇。酿酒酵母首先消耗葡萄糖，然后消耗半乳糖。评估了同时消耗葡萄糖和半乳糖以减少发酵时间和提高乙醇产率系数的方法。酿酒酵母的 Gal3p 和 Gal80p 作为信号转导物，控制半乳糖诱导物 Gal4p 对半乳糖基因家族的转录激活。Gal80p 与 Gal4p 结合以失活转录。因此，为了不中断 Gal4p 的表达，删除了 Gal80p。