Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania.
Department of Diagnostic and Interventional Radiology, Osaka City University, Osaka-shi, Osaka, Japan.
Curr Protoc. 2021 Apr;1(4):e110. doi: 10.1002/cpz1.110.
The propensity of uveal melanoma to metastasize to the liver hinders the accrual of micro-metastatic and end-stage disease tissue samples and restricts the investigation of metastatic uveal melanoma (MUM). Pre-clinical experimental animal models of MUM can help elucidate the pathophysiology of metastatic lesions and provide a tool for designing new therapeutic approaches for MUM. Here, we present an advanced model of hepatic metastases that enables quantitatively visualizing the development of individual hepatic tumor clones and estimating their growth kinetics and colonization efficiency. Similar to clinically observed liver metastases, these models enable the assessment of growth kinetics of the liver micro-metastases and the testing of therapeutic approaches for the treatment of MUM. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Experimental patient-derived xenograft mouse model of metastatic uveal melanoma Basic Protocol 2: Experimental liver micro-metastatic mouse model using splenic injection of metastatic uveal melanoma cells.
葡萄膜黑色素瘤向肝脏转移的倾向阻碍了微转移和终末期疾病组织样本的积累，并限制了对转移性葡萄膜黑色素瘤（MUM）的研究。MUM 的临床前实验动物模型可以帮助阐明转移病变的病理生理学，并为设计治疗 MUM 的新方法提供工具。在这里，我们提出了一种先进的肝转移模型，能够定量观察单个肝肿瘤克隆的发展，并估计其生长动力学和定植效率。与临床上观察到的肝转移类似，这些模型能够评估肝微转移的生长动力学，并测试治疗 MUM 的方法。© 2021 年 Wiley 期刊 LLC。 基本方案 1：转移性葡萄膜黑色素瘤的实验性患者来源异种移植小鼠模型 基本方案 2：使用转移性葡萄膜黑色素瘤细胞脾脏注射的实验性肝微转移小鼠模型。
