氨基葡萄糖可减轻米托蒽醌耐药乳腺癌细胞的耐药性。

Glucosamine attenuates drug resistance in Mitoxantrone-resistance breast cancer cells.

机构信息

Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad,Iran.

出版信息

J Pharm Pharmacol. 2021 Jun 8;73(7):922-927. doi: 10.1093/jpp/rgaa032.

DOI:10.1093/jpp/rgaa032

PMID:33885909

Abstract

OBJECTIVES

This study was aimed at investigating the cytotoxicity and multi-drug resistance (MDR) reversal effect of Glucosamine (GlcN) on resistant BCRP-overexpressing breast cancer MCF-7/MX cells.

METHODS

After confirming the overexpression of BCRP, the cytotoxicity and MDR reversing potential of GlcN on MCF-7/MX mitoxantrone-resistant and MCF-7 sensitive breast cancer cells were assessed via MTT assay. The effects of GlcN on mitoxantrone accumulation were analyzed through flow cytometry. Finally, the expression of BCRP and Epithelial-Mesenchymal Transition (EMT)-related markers following the exposure to GlcN were assessed by real-time RT-PCR.

KEY FINDINGS

This study showed that glucosamine had an inhibitory effect on the proliferation of human breast cancer cells. The respective IC50 values for MCF-7/MX cells following exposure to mitoxantrone (MX) in the presence of GlcN (0, 0.5 and 1 mm) for 72 h were 3.61 ± 0.21, 0.598 ± 0.041 and 0.284 ± 0.016 μm, respectively. Furthermore, GlcN reduced the expression of BCRP mRNA without any significant effect on EMT-related markers in breast cancer cells.

CONCLUSIONS

These results proposed that glucosamine as a natural sugar could down regulate the BCRP expression and increased MX cytotoxicity in breast cancer cells.

摘要

目的

本研究旨在探讨氨基葡萄糖（GlcN）对耐药 BCRP 过表达乳腺癌 MCF-7/MX 细胞的细胞毒性和多药耐药（MDR）逆转作用。

方法

在确认 BCRP 过表达后，通过 MTT 法评估 GlcN 对 MCF-7/MX 米托蒽醌耐药和 MCF-7 敏感乳腺癌细胞的细胞毒性和 MDR 逆转潜力。通过流式细胞术分析 GlcN 对米托蒽醌蓄积的影响。最后，通过实时 RT-PCR 评估暴露于 GlcN 后 BCRP 和上皮-间充质转化（EMT）相关标志物的表达。

主要发现

本研究表明氨基葡萄糖对人乳腺癌细胞的增殖具有抑制作用。在存在 GlcN（0、0.5 和 1mm）的情况下，MCF-7/MX 细胞暴露于米托蒽醌（MX）72 小时后的各自 IC50 值分别为 3.61±0.21、0.598±0.041 和 0.284±0.016μm。此外，GlcN 降低了 BCRP mRNA 的表达，而对乳腺癌细胞中 EMT 相关标志物没有任何显著影响。