Biotechnology Division, ICAR-Indian Institute of Rice Research (IIRR), Hyderabad 500030, India.
Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India.
Gene. 2021 Jul 15;789:145670. doi: 10.1016/j.gene.2021.145670. Epub 2021 Apr 20.
We have functionally characterized the RPL6, a Ribosomal Protein Large subunit gene for salt stress tolerance in rice. The overexpression of RPL6 resulted in tolerance to moderate (150 mM) to high (200 mM) levels of salt (NaCl). The transgenic rice plants expressing RPL6 constitutively showed better phenotypic and physiological responses with high quantum efficiency, accumulation of higher chlorophyll and proline contents, and an overall increase in seed yield compared with the wild type in salt stress treatments. An iTRAQ-based comparative proteomic analysis revealed the high expression of about 333 proteins among the 4378 DAPs in a selected overexpression line of RPL6 treated with 200 mM of NaCl. The functional analysis showed that these highly accumulated proteins (HAPs) are involved in photosynthesis, ribosome and chloroplast biogenesis, ion transportation, transcription and translation regulation, phytohormone and secondary metabolite signal transduction. An in silico network analysis of HAPs predicted that RPL6 binds with translation-related proteins and helicases, which coordinately affect the activities of a comprehensive signaling network, thereby inducing tolerance and promoting growth and productivity in response to salt stress. Our overall findings identified a novel candidate, RPL6, whose characterization contributed to the existing knowledge on the complexity of salt tolerance mechanism in plants.
我们对 RPL6（核糖体大亚基基因）进行了功能表征，该基因可增强水稻的耐盐性。过表达 RPL6 可使水稻耐受中等（150 mM）至高（200 mM）盐浓度（NaCl）胁迫。与野生型相比，持续表达 RPL6 的转基因水稻在盐胁迫处理中表现出更好的表型和生理响应，具有更高的量子效率、更高的叶绿素和脯氨酸含量积累，以及整体种子产量增加。基于 iTRAQ 的比较蛋白质组学分析显示，在经 200 mM NaCl 处理的 RPL6 过表达系中，在 4378 个 DAPs 中约有 333 个蛋白高表达。功能分析表明，这些高丰度蛋白（HAPs）参与光合作用、核糖体和叶绿体生物发生、离子转运、转录和翻译调控、植物激素和次生代谢物信号转导。HAPs 的计算机网络分析预测，RPL6 与翻译相关蛋白和解旋酶结合，共同影响全面信号网络的活性，从而诱导耐盐性，并在应对盐胁迫时促进生长和生产力。我们的总体研究结果确定了一个新的候选基因 RPL6，其鉴定有助于增加对植物耐盐机制复杂性的现有认识。
