严重肥胖症患者内脏和皮下脂肪组织中脂肪细胞蛋白与内源性脂肪酸的储存。
Adipocyte Proteins and Storage of Endogenous Fatty Acids in Visceral and Subcutaneous Adipose Tissue in Severe Obesity.
机构信息
Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA.
Department of Surgery, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
Obesity (Silver Spring). 2021 Jun;29(6):1014-1021. doi: 10.1002/oby.23149. Epub 2021 Apr 24.
OBJECTIVE
This study tested whether substrate concentrations or fatty acid storage proteins predict storage of endogenous lipids in visceral adipose tissue (VAT) and upper body subcutaneous adipose tissue (UBSQ) fat.
METHODS
The day prior to surgery, 25 patients undergoing bariatric procedures received an infusion of autologous [1- C]triolein-labeled very low-density lipoprotein (VLDL) particles, and during surgery, they received a continuous [U- C]palmitate infusion/bolus [9,10- H]palmitate tracer. VAT and UBSQ fat were collected to measure VLDL-triglyceride (TG) storage, direct free fatty acid (FFA) storage rates, CD36 content, lipoprotein lipase (LPL), acyl-CoA synthetase, diacylglycerol acetyl-transferase, and glycerol-3-phosphate acyltransferase activities.
RESULTS
Storage of VLDL-TG and FFA-palmitate in UBSQ and VAT was not different. Plasma palmitate concentrations correlated with palmitate storage rates in UBSQ and VAT (r = 0.46, P = 0.02 and r = 0.46, P = 0.02, respectively). In VAT, VLDL-TG storage was correlated with VLDL concentrations (r = 0.53, P < 0.009) and LPL (r = 0.42, P < 0.05). In UBSQ, VLDL-TG storage was correlated with LPL (r = 0.42, P < 0.05). CD36, acyl-CoA synthetase, glycerol-3-phosphate acyltransferase, and diacylglycerol acetyl-transferase were not correlated with VLDL-TG or palmitate storage.
CONCLUSIONS
Adipose storage of VLDL-TG is predicted by VLDL-TG concentrations and LPL; FFA concentrations predict direct adipose tissue FFA storage rates.
目的
本研究旨在探讨基质浓度或脂肪酸储存蛋白是否能预测内脏脂肪组织（VAT）和上半身皮下脂肪组织（UBSQ）脂肪中内源性脂质的储存情况。
方法
在手术前一天，25 名接受减重手术的患者接受了自体[1-¹³C]三油精标记的极低密度脂蛋白（VLDL）颗粒输注，手术期间接受了持续的[U-¹³C]棕榈酸输注/棕榈酸[9,10-³H]示踪剂脉冲。收集 VAT 和 UBSQ 脂肪以测量 VLDL-甘油三酯（TG）储存、直接游离脂肪酸（FFA）储存率、CD36 含量、脂蛋白脂肪酶（LPL）、酰基辅酶 A 合成酶、二酰基甘油乙酰转移酶和甘油-3-磷酸酰基转移酶活性。
结果
UBSQ 和 VAT 中 VLDL-TG 和 FFA-棕榈酸的储存没有差异。血浆棕榈酸浓度与 UBSQ 和 VAT 中棕榈酸储存率相关（r = 0.46，P = 0.02 和 r = 0.46，P = 0.02）。在 VAT 中，VLDL-TG 储存与 VLDL 浓度（r = 0.53，P < 0.009）和 LPL（r = 0.42，P < 0.05）相关。在 UBSQ 中，VLDL-TG 储存与 LPL 相关（r = 0.42，P < 0.05）。CD36、酰基辅酶 A 合成酶、甘油-3-磷酸酰基转移酶和二酰基甘油乙酰转移酶与 VLDL-TG 或棕榈酸储存无关。
结论
VLDL-TG 的脂肪储存由 VLDL-TG 浓度和 LPL 预测；FFA 浓度预测直接脂肪组织 FFA 储存率。
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