Mizzou Institute for Women's Health Research, Department of Obstetrics, Gynecology and Women's Health, University of Missouri School of Medicine, Columbia, MO USA.
Bond Life Sciences Center, Division of Animal Sciences, University of Missouri, Columbia, MO USA.
Biol Reprod. 2021 Jul 2;105(1):40-51. doi: 10.1093/biolre/ioab080.
It is very difficult to gain a better understanding of the events in human pregnancy that occur during and just after implantation because such pregnancies are not yet clinically detectable. Animal models of human placentation are inadequate. In vitro models that utilize immortalized cell lines and cells derived from trophoblast cancers have multiple limitations. Primary cell and tissue cultures often have limited lifespans and cannot be obtained from the peri-implantation period. We present here two contemporary models of human peri-implantation placental development: extended blastocyst culture and stem-cell derived trophoblast culture. We discuss current research efforts that employ these models and how such models might be used in the future to study the "black box" stage of human pregnancy.
要想更好地了解着床期间及刚刚着床后发生的人类妊娠事件非常困难，因为此时妊娠还无法在临床上检测到。人类胎盘形成的动物模型并不完善。利用永生化细胞系和滋养层癌细胞衍生细胞的体外模型存在多种局限性。原代细胞和组织培养物的寿命通常有限，并且无法在着床期获得。我们在此介绍两种当代人类着床期胎盘发育模型：扩展囊胚培养和干细胞衍生滋养层培养。我们讨论了当前使用这些模型的研究工作，以及这些模型将来如何用于研究人类妊娠的“黑箱”阶段。
