Terrestrial ecosystems are the core components of the biosphere, supporting human life, production and social and economic activities. With the development of human civilization and the progress of science and technology, the continued expanding resources utilization in terms of scale and intensity has caused environmental problems, including global climate change, biodiversity loss, environmental pollution, resource depletion and ecosystem degradation, which threaten the sustainable development of our society. The public expects ecological research providing scientific theories and systematic solutions for the utilization and protection of ecosystems at the continental and global scale, and the maintenance of sustainable development of human society as well. This study aimed to serve the ecological civilization construction in China, ecological security pattern construction, regional ecological and environmental governance, and the development of macrosystem ecology. We reviewed the effectiveness and experience of regional ecological and environmental governance in China, analyzed the scientific requirements and the characteristics of the times of China's eco-civilization construction towards large-scale ecological research, and proposed using a new philosophy, ., nature-based macroecosystem approach, to improve the ecological and environmental governance and ecological security pattern construction at the regional scale. Based on the above-mentioned expounding, we discussed the strategic plan, technical approaches and technological support system of large-scale ecological security pattern construction and ecological and environmental governance in China. This review could provide theoretical references for safe, healthy and beautiful land spatial construction and the progress of eco-civilization of China.