Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.
Monoclon Antib Immunodiagn Immunother. 2021 Apr;40(2):76-80. doi: 10.1089/mab.2021.0008.
Killer cell lectin-like receptor subfamily G member 1 (KLRG1), a type II transmembrane protein, was identified as an inhibitory receptor expressed on natural killer (NK) cells and certain T cells. The protein regulates effector functions and developmental processes in these cells. In this study, we established a specific and sensitive monoclonal antibody (mAb) for human KLRG1 (hKLRG1), which is useful for flow cytometry, using a Cell-Based Immunization and Screening (CBIS) method. The established anti-hKLRG1 mAb, KLMab-1 (mouse IgG, kappa), reacted with overexpressed hKLRG1 in Chinese hamster ovary-K1 (CHO/hKLRG1) and human NK cells, which also expressed endogenous hKLRG1 as confirmed by flow cytometry. KLMab-1, which was established by the CBIS method, could be useful for elucidating the hKLRG1-related biological response by flow cytometry.
杀伤细胞凝集素样受体亚家族 G 成员 1（KLRG1）是一种 II 型跨膜蛋白，被鉴定为自然杀伤（NK）细胞和某些 T 细胞上表达的抑制性受体。该蛋白调节这些细胞的效应功能和发育过程。在这项研究中，我们使用基于细胞的免疫接种和筛选（CBIS）方法，建立了一种针对人 KLRG1（hKLRG1）的特异性和灵敏性单克隆抗体（mAb），该 mAb 可用于流式细胞术。所建立的抗 hKLRG1 mAb，KLMab-1（鼠 IgG，kappa），通过流式细胞术证实，与过表达的中国仓鼠卵巢-K1（CHO/hKLRG1）和人 NK 细胞中的 hKLRG1 反应，CHO/hKLRG1 和人 NK 细胞中也表达内源性 hKLRG1。通过 CBIS 方法建立的 KLMab-1 可用于通过流式细胞术阐明 hKLRG1 相关的生物学反应。
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