Beijing Youan Hospital of Capital Medical University, Fengtai District, Beijing China.
Beijing Chaoyang Hospital of Capital Medical University, Chaoyang District, Beijing China.
Am J Mens Health. 2021 Mar-Apr;15(2):15579883211011380. doi: 10.1177/15579883211011380.
To study the differential gene expression and clinical significance in human immunodeficiency virus-infected individuals (HIVIIs) with penile squamous cell carcinoma. At our hospital from 2019 to 2020, we selected six samples of HIV-related penile squamous cell carcinoma for the experimental group and six samples of non-HIV-related penile squamous cell carcinoma for the control group. Transcriptome sequencing of sample mRNAs was performed by high-throughput sequencing. Differential gene expression analysis, differential Gene Ontology (GO) enrichment analysis and differential Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out, and the reads per kilobase per million reads (RPKM) value was used as a measure of gene expression. A total of 2418 differentially expressed genes were obtained, of which 663 were upregulated and 1755 were downregulated (absolute value of logFC >1 and value <.05). On the basis of the significance of the GO enrichment analysis, we found that the tumor protein p63 (TP63) gene was significantly upregulated and that the LIM domain only 4 (LMO4) gene was significantly downregulated in the experimental group compared with the control group. KEGG pathway analysis of the differentially expressed genes revealed that DNA replication was the most significant pathway associated with the upregulated genes and cell adhesion molecule (CAM) metabolism was the most significant pathway associated with the downregulated genes. The gene expression profiles of HIV-related penile squamous cell carcinoma and non-HIV-related penile squamous cell carcinoma are significantly different and involve significant GO enrichment and KEGG metabolic pathways, and this is very meaningful for the study of non-AIDS-defining cancers (NADCs). Differential expression of genes may be an important target for the prevention of penile squamous cell carcinoma in HIVIIs.
研究人类免疫缺陷病毒感染个体（HIVIIs）中阴茎鳞状细胞癌的差异基因表达和临床意义。在我院 2019 年至 2020 年期间，选择 6 例 HIV 相关阴茎鳞状细胞癌样本作为实验组，6 例非 HIV 相关阴茎鳞状细胞癌样本作为对照组。通过高通量测序对样本 mRNA 进行转录组测序。进行差异基因表达分析、差异基因本体论（GO）富集分析和差异京都基因与基因组百科全书（KEGG）通路分析，并用每千碱基每百万读段（RPKM）值作为基因表达的衡量标准。共获得 2418 个差异表达基因，其中 663 个上调，1755 个下调（绝对值 logFC >1 和 值 <.05）。基于 GO 富集分析的显著性，我们发现实验组中肿瘤蛋白 p63（TP63）基因显著上调，LIM 结构域只有 4（LMO4）基因显著下调。差异表达基因的 KEGG 通路分析表明，DNA 复制是与上调基因最显著相关的途径，细胞黏附分子（CAM）代谢是与下调基因最显著相关的途径。HIV 相关阴茎鳞状细胞癌和非 HIV 相关阴茎鳞状细胞癌的基因表达谱存在显著差异，涉及显著的 GO 富集和 KEGG 代谢途径，这对于非艾滋病定义癌症（NADCs）的研究非常有意义。基因的差异表达可能是预防 HIVIIs 阴茎鳞状细胞癌的一个重要靶点。
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