多重聚合酶链反应在热带国家感染性腹泻诊断中的应用

The utility of multiplex polymerase chain reaction for diagnosis of infectious diarrhoea in a tropical country.

作者信息

Ghoshal Ujjala, Tejan Nidhi, Sisodia Juhi, Verma Shikha, Prasad Narayan, Uc Ghoshal

机构信息

Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Raebareli Road, Lucknow, 226014, India.

出版信息

Indian J Med Microbiol. 2021 Jul;39(3):323-327. doi: 10.1016/j.ijmmb.2021.03.024. Epub 2021 Apr 24.

DOI:10.1016/j.ijmmb.2021.03.024

PMID:33906747

Abstract

PURPOSE

The etiology of infective diarrheaoften remains undiagnosed. We studied the role of multiplex polymerase chain reaction (PCR) for detection of etiological agents of diarrhoea.

METHODS

Fast track diagnostics (FTD)gastroenteritis panel for bacterial and viral pathogens was used to test stool samples from patients with diarrhoea.

RESULTS

Stool samples from 276 patients (138 immunocompetent and 138 immunocompromised) with diarrhoea and 138 healthy controls were tested. Bacterial culture was positive in 5 samples. Following agents were isolated: Shigella sonnei(2), Shigella dysentriae(1), SalmonellaParatyphi B(1) and Vibrio cholerae (1). Multiplex PCR panel did not include Vibrio cholerae in its panel. A total of 65 target pathogens were identified in 60/276 (21.7%) patients by multiplex PCR. 28/65(41.1%) and 37/65 (56.9%) were bacterial and viral agents respectively. Identified bacteria were Shigella(20), Salmonella(3), Campylobacter(4) and Clostridium difficile(1). Viral targets identified were Norovirus GII (28), Adenovirus(4), Astrovirus(3) and Sapovirus(2). All the controls were negative for enteropathogens by conventional methods and multiplex PCR.

CONCLUSIONS

Our detection rates increased from 1.8% (5/276)by conventional methods to 21.7% (60/276)by multiplex PCR, which included both bacterial as well as viral targets.

摘要

目的

感染性腹泻的病因常常难以确诊。我们研究了多重聚合酶链反应（PCR）在检测腹泻病原体中的作用。

方法

采用快速诊断（FTD）肠胃炎检测板检测腹泻患者的粪便样本中的细菌和病毒病原体。

结果

对276例腹泻患者（138例免疫功能正常者和138例免疫功能低下者）以及138例健康对照者的粪便样本进行了检测。细菌培养有5份样本呈阳性。分离出的病原体如下：宋内志贺菌（2株）、痢疾志贺菌（1株）、副伤寒沙门菌B（1株）和霍乱弧菌（1株）。多重PCR检测板中未包含霍乱弧菌。通过多重PCR在60/276（21.7%）例患者中总共鉴定出65种目标病原体。其中分别有28/65（41.1%）和37/65（56.9%）为细菌和病毒病原体。鉴定出的细菌有：志贺菌（20株）、沙门菌（3株）、弯曲杆菌（4株）和艰难梭菌（1株）。鉴定出的病毒目标有：诺如病毒GII（28株）、腺病毒（4株）、星状病毒（3株）和札如病毒（2株）。所有对照者通过传统方法和多重PCR检测肠道病原体均为阴性。