Al-Shudifat Anas Mohammed, Al-Shahwan Ibrahim Mohammed, Al-Saleh Mohammad Ali, Abdalla Omer Ahmed, Amer Mahmoud Ahmed
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Apr;28(4):2360-2365. doi: 10.1016/j.sjbs.2021.01.031. Epub 2021 Jan 28.
A survey was conducted in Al-Kharj governorate, Riyadh region to identify viruses causing variety of virus-like symptoms on tomato plants. A total of 135 samples were collected from symptomatic tomato plants. Symptoms included mottling, deformation, necrosis of leaves and fruits. Eighteen viruses were tested by DAS-ELISA. (TBRV) was the virus of concern as it was not detected in Saudi Arabia before and was detected in 52.6% of the collected samples in this study. RT-PCR was used to confirm detection of TBRV and to sequence the amplified products to determine molecular characteristics of this virus. In the host range test study that was performed using a purified isolate of TBRV, sixteen out of the twenty two tested plants showed symptoms. was not infected by this virus. Gel electrophoreses (2% agarose) yielded fragments of 978 bp of coat protein gene of TBRV. Nucleotide sequences of purified RT-PCR products for three TBRV Saudi isolates were deposited in the GenBank with the following accession numbers MT274656, MT274657, and MT274658. These isolates of TBRV indicated a close Phylogenetic relationship of (99-100%) among themselves and with five isolates from Poland (95-98%) but a distant relationship of 85% with isolates from England and Lithuania deposited in the GenBank. This is the first report for detection and molecular characterization of TBRV infecting tomato plants in Saudi Arabia.
在利雅得地区的哈吉尔省开展了一项调查，以确定导致番茄植株出现各种病毒样症状的病毒。从出现症状的番茄植株上共采集了135个样本。症状包括叶片和果实出现斑驳、变形、坏死。通过双抗夹心酶联免疫吸附测定法（DAS-ELISA）对18种病毒进行了检测。番茄褐纹病毒（TBRV）是令人关注的病毒，因为此前在沙特阿拉伯未检测到该病毒，而在本研究采集的样本中有52.6%检测到了该病毒。采用逆转录聚合酶链反应（RT-PCR）来确认TBRV的检测结果，并对扩增产物进行测序，以确定该病毒的分子特征。在使用纯化的TBRV分离株进行的寄主范围测试研究中，22株受试植物中有16株出现了症状。未被这种病毒感染。凝胶电泳（2%琼脂糖）产生了TBRV外壳蛋白基因978 bp的片段。三个沙特TBRV分离株的纯化RT-PCR产物的核苷酸序列已存入GenBank，登录号分别为MT274656、MT274657和MT274658。这些TBRV分离株表明，它们彼此之间以及与来自波兰的五个分离株（95 - 98%）具有密切的系统发育关系（99 - 100%），但与GenBank中来自英国和立陶宛的分离株的关系较远，为85%。这是沙特阿拉伯首次关于感染番茄植株的TBRV的检测和分子特征的报告。
