韩国马疱疹病毒1型中ORF34、ORF68和多位点序列分型基因的变异性评估

Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea.

作者信息

Kang Hyung-Woo, Lee Eun-Yong, Lee Kyoung-Ki, Ko Mi-Kyeong, Park Ji-Young, Kim Yeon-Hee, Lee Kyunghyun, Choi Eun-Jin, Kim Jongho, So ByungJae, Park Choi-Kyu, Jeoung Hye-Young

机构信息

Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.

College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Korea.

出版信息

Pathogens. 2021 Apr 2;10(4):425. doi: 10.3390/pathogens10040425.

DOI:10.3390/pathogens10040425

PMID:33918404

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8066002/

Abstract

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7-100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701-818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.

摘要

马疱疹病毒1型（EHV-1）是马匹中的一种重要病原体。它在全球范围内影响马匹，并造成重大经济损失。在本研究中，我们首次在分子水平上对来自韩国的EHV-1分离株进行了特征分析。然后，我们旨在通过将这些分离株与数据库中的序列进行比较来确定它们的遗传差异。总共收集了338份马样本，分离出12株EHV-1。我们对12株分离的EHV-1进行了ORF30、ORF33、ORF68和ORF34基因分析，并进行了多位点序列分型（MLST）。所有分离出的病毒均被确认为非神经致病性类型，显示ORF30的N752和高度保守的ORF33（99.7-100%）。由于核苷酸序列701-818中存在118 bp的缺失，使用ORF68分析无法对分离株进行分类。根据ORF34和MLST分析，7株EHV-1分离株（16Q4、19R166-1、19R166-6、19/10/15-2、19/10/15-4、19/10/18-2、19/10/22-1）属于第1组，第10进化枝。其余5株EHV-1分离株（15Q25-1、15D59、16Q5、16Q40、18D99）根据ORF34和MLST分析属于第7组，第6进化枝。