Storms Julia, Wirth Anna, Vasiliadis Danae, Brodard Isabelle, Hamann-Thölken Antje, Ambros Christina, Moog Udo, Jores Jörg, Kuhnert Peter, Distl Ottmar
Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), 30559 Hannover, Germany.
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.
Animals (Basel). 2021 Apr 12;11(4):1102. doi: 10.3390/ani11041102.
The bacterium () is the causative agent of ovine footrot. The aim of this field study was to determine the prevalence of in German sheep flocks. The sheep owners participated voluntarily in the study. More than 9000 sheep from 207 flocks were screened for footrot scores using a Footrot Scoring System from 0 to 5 and sampling each sheep using one interdigital swab for all four feet of the sheep. The detection and discrimination between benign and virulent strains was done employing a real-time PCR. Our results showed a mean prevalence of 42.93% of in German sheep on an animal level. Underrunning of hoof horn on at least one foot (Scores 3-5) was detected in 567 sheep (6.13%). Sheep with four clinically healthy feet were found through visual inspection in 47.85% of all animals included in this study. In total, 1117 swabs from sheep with four clinically healthy feet tested positive for . In 90.35% of the positive swabs, virulent were detected. Benign were detected in 4.74% of the -positive swabs while 4.91% tested positive for both, benign and virulent . In 59 flocks were not detected and in 115 flocks only virulent were found while seven flocks tested positive for benign strains.
细菌（）是绵羊腐蹄病的病原体。本田间研究的目的是确定德国羊群中该细菌的流行率。羊主自愿参与了该研究。使用从0到5的腐蹄病评分系统对来自207个羊群的9000多只羊进行腐蹄病评分筛查，并对每只羊的四只脚各用一个趾间拭子进行采样。采用实时聚合酶链反应进行良性和毒性菌株的检测与鉴别。我们的结果显示，在动物层面上，德国绵羊中该细菌的平均流行率为42.93%。在567只羊（6.13%）中检测到至少一只脚有蹄角质下翻（评分3 - 5）。通过目视检查，在本研究纳入的所有动物中，47.85%的羊四只脚临床健康。总共，来自四只脚临床健康的羊的1117份拭子检测出该细菌呈阳性。在90.35%的阳性拭子中检测到毒性菌株。在4.74%的该细菌阳性拭子中检测到良性菌株，而4.91%的拭子同时检测出良性和毒性菌株呈阳性。在59个羊群中未检测到该细菌，在115个羊群中仅发现毒性菌株，而7个羊群的良性菌株检测呈阳性。
