do Espírito Santo Júnior José, Gabrielle Ramos de Mesquita Tirza, Diego Oliveira da Silva Luan, Jules de Araújo Felipe, Lacerda de Souza Josué, Carneiro de Lacerda Thaís, Santos da Silva Lener, Marcello da Silveira Júnior Cláudio, Layane Guimarães Duarte Queiroz Krys, Dos Santos Diogo Matos, Chagas da Silva Cilana, David Graterol Sequera Héctor, Tamayo Hermida Melissa, Lúcia Gomes de Souza Mara, Vinitius de Farias Guerra Marcus, Ramasawmy Rajendranath
Programa de Pós-Graduação em Imunologia Básica e Aplicada, Universidade Federal do Amazonas, Manaus 69080-900, AM, Brazil.
Faculdade de Medicina Nilton Lins, Universidade Nilton Lins, Manaus 69058-030, AM, Brazil.
Pathogens. 2021 Apr 20;10(4):498. doi: 10.3390/pathogens10040498.
BACKGROUND: Leishmaniasis is an infectious disease caused by parasites. A Th1 immune response is necessary in the acute phase to control the pathogen. The triggering receptor expressed on myeloid cells (TREM)-1 is a potent amplifier of inflammation. Our aim is to identify whether the variant rs2234237 A/T (Thr25Ser) is associated with the disease development of cutaneous leishmaniasis (CL) in -infected individuals. The effects of the rs2234237 genotypes on plasma cytokines IL-1β, IL-6, IL-8, IL-10, MCP-1 and TNF-α are also investigated. METHODS: 838 patients with CL and 818 healthy controls (HCs) living in the same endemic areas were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Plasma cytokines were assayed in 400 patients with CL and 400 HCs using the BioPlex assay. RESULTS: The genotypes' and alleles' frequencies were similar in both patients with CL (AA = 618, 74%; AT = 202, 24%; TT = 18, 2%) and in HCs (AA = 580, 71%; AT = 220, 27%; TT = 18, 2%). Rs2234237 showed a modest effect on plasma IL-10 that disappeared when correction of the -value was applied. Plasma IL-10 by rs2234237 genotypes were (mean ± SEM; AA = 2.91 pg/mL ± 0.14; AT = 2.35 pg/mL ± 0.12; TT = 3.14 pg/mL ± 0.56; = 0.05). CONCLUSION: The rs2234237 (Thr25Ser) seems to have no influence on the susceptibility or resistance to infections.
背景：利什曼病是一种由寄生虫引起的传染病。急性期需要Th1免疫反应来控制病原体。髓系细胞触发受体（TREM）-1是炎症的强效放大器。我们的目的是确定rs2234237 A/T（Thr25Ser）变体是否与皮肤利什曼病（CL）感染个体的疾病发展相关。还研究了rs2234237基因型对血浆细胞因子白细胞介素（IL）-1β、IL-6、IL-8、IL-10、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）的影响。 方法：采用聚合酶链反应-限制性片段长度多态性方法对838例居住在同一流行地区的CL患者和818例健康对照（HC）进行基因分型。使用BioPlex检测法对400例CL患者和400例HC的血浆细胞因子进行检测。 结果：CL患者（AA = 618，74%；AT = 202，24%；TT = 18，2%）和HC（AA = 580，71%；AT = 220，27%；TT = 18，2%）的基因型和等位基因频率相似。rs2234237对血浆IL-10有适度影响，在校正P值后这种影响消失。rs2234237基因型的血浆IL-10水平为（平均值±标准误；AA = 2.91 pg/mL±0.14；AT = 2.35 pg/mL±0.12；TT = 3.14 pg/mL±0.56；P = 0.05）。 结论：rs2234237（Thr25Ser）似乎对感染的易感性或抵抗力没有影响。
Front Cell Infect Microbiol. 2019-5-2
Lancet. 2018-8-17
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