Reidy M A
Department of Pathology, University of Washington, Seattle.
Lab Invest. 1988 Jul;59(1):36-43.
This study examined the ability of arterial endothelial cells to repopulate arterial grafts which were devoid of luminal smooth muscle cells and in arterial grafts which had an established pseudoendothelium comprised of smooth muscle cells. These studies were designed to explore the hypothesis that smooth muscle cells when forming a pseudoendothelium can retard endothelial cell regrowth. Segments of denuded glutaraldehyde-fixed carotid arteries and of viable autologous carotid arteries with a pseudoendothelium of smooth muscle cells were implanted end to end into the rabbit carotid arteries and left for 6 or 12 weeks. Endothelial outgrowth onto these arterial grafts was extremely limited (approximately 3 mm) regardless of the preparation and was equal to that observed in a carotid artery denuded of endothelium with a balloon catheter. Endothelial cell replication was significantly elevated in these implanted carotid segments at all times studied with the majority of the replicating cells not located at the leading edge of endothelium. The location of these cells suggests this replication was not associated with regrowth. Finally, the ability of denuded arteries to support endothelial cell adherence was tested in vitro by plating [3H]thymidine bovine endothelial cells on segments of excised arteries which had been denuded of endothelium for 2 and 12 weeks. No difference in plating efficiency was observed. These studies suggest that the presence of smooth muscle cells on the luminal surface of denuded arteries was not responsible for the cessation of endothelial regrowth.
本研究检测了动脉内皮细胞在腔内无平滑肌细胞的动脉移植物以及已形成由平滑肌细胞构成的假内皮的动脉移植物中重新填充的能力。这些研究旨在探讨平滑肌细胞形成假内皮时会阻碍内皮细胞再生这一假说。将戊二醛固定的去内皮颈动脉段以及带有平滑肌细胞假内皮的自体活颈动脉段端端植入兔颈动脉，留置6周或12周。无论制备方式如何，这些动脉移植物上的内皮生长都极其有限（约3毫米），且与用球囊导管去除内皮的颈动脉中观察到的情况相当。在所有研究时间点，这些植入的颈动脉段中内皮细胞复制均显著增加，大多数复制细胞并不位于内皮的前沿。这些细胞的位置表明这种复制与再生无关。最后，通过将[³H]胸腺嘧啶核苷标记的牛内皮细胞接种在已去除内皮2周和12周的离体动脉段上，在体外测试去内皮动脉支持内皮细胞黏附的能力。未观察到接种效率有差异。这些研究表明，去内皮动脉腔表面平滑肌细胞的存在并非内皮再生停止的原因。
