Institute for Advanced Study, Chengdu University, China.
Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin, National Ethnic Affairs Commission of the People's Republic of China, College of Biological Science & Engineering, North Minzu University, Yinchuan, China.
Genome Biol Evol. 2021 Jun 8;13(6). doi: 10.1093/gbe/evab094.
Jacaranda mimosifolia D. Don is a deciduous tree widely cultivated in the tropics and subtropics of the world. It is famous for its beautiful blue flowers and pinnate compound leaves. In addition, this tree has great potential in environmental monitoring, soil quality improvement, and medicinal applications. However, a genome resource for J. mimosifolia has not been reported to date. In this study, we constructed a chromosome-level genome assembly of J. mimosifolia using PacBio sequencing, Illumina sequencing, and Hi-C technology. The final genome assembly was ∼707.32 Mb in size, 688.76 Mb (97.36%) of which could be grouped into 18 pseudochromosomes, with contig and scaffold N50 values of 16.77 and 39.98 Mb, respectively. A total of 30,507 protein-coding genes were predicted, 95.17% of which could be functionally annotated. Phylogenetic analysis among 12 plant species confirmed the close genetic relationship between J. mimosifolia and Handroanthus impetiginosus. Gene family clustering revealed 481 unique, 103 significantly expanded, and 16 significantly contracted gene families in the J. mimosifolia genome. This chromosome-level genome assembly of J. mimosifolia will provide a valuable genomic resource for elucidating the genetic bases of the morphological characteristics, adaption evolution, and active compounds biosynthesis of J. mimosifolia.
蓝花楹是一种落叶乔木，广泛分布于世界热带和亚热带地区。它以美丽的蓝色花朵和羽状复叶而闻名。此外，这种树在环境监测、土壤质量改善和药用应用方面具有巨大的潜力。然而，到目前为止，还没有关于蓝花楹基因组资源的报道。在这项研究中，我们使用 PacBio 测序、Illumina 测序和 Hi-C 技术构建了蓝花楹的染色体水平基因组组装。最终的基因组组装大小约为 707.32Mb，其中 688.76Mb（97.36%）可以分组到 18 条假染色体中，其 contig 和 scaffold N50 值分别为 16.77Mb 和 39.98Mb。总共预测到 30507 个蛋白质编码基因，其中 95.17%可以进行功能注释。在 12 种植物物种的系统发育分析中，证实了蓝花楹与 Handroanthus impetiginosus 之间的密切遗传关系。基因家族聚类显示，在蓝花楹基因组中存在 481 个独特、103 个显著扩张和 16 个显著收缩的基因家族。蓝花楹的染色体水平基因组组装将为阐明其形态特征、适应进化和活性化合物生物合成的遗传基础提供有价值的基因组资源。
