Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain; Instituto de Investigación Química "Andrés M. del Rio" (IQAR), Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
Food Chem. 2021 Oct 15;359:129874. doi: 10.1016/j.foodchem.2021.129874. Epub 2021 Apr 28.
A green methodology based on pressurized liquids (PLE) to extract proteins and obtain highly active extracts from brewer's spent grain (BSG) is proposed. Box-Behnken experimental design was employed to study the effect of extraction parameters on the protein content (PC), the total phenolic content (TPC), and the antioxidant activity of extracts. Results were compared with those obtained by conventional alkaline extraction assisted with ultrasounds (UAE). The selection of PLE conditions enabled to tailor the PC and TPC of extracts. PLE extracted 36 % more proteins than UAE. PLE extracts showed higher antioxidant, cholesterol esterase inhibition, and ACE inhibitory activities than UAE extract. HPLC-MS/MS enabled to observe that the extraction technique and experimental conditions significantly affected to the kind and amount of extracted proteins, and released peptides, and phenolic compounds. A higher ratio of hydrophobic peptides was observed in PLE extracts, which justified their higher bioactivity.
提出了一种基于加压液体（PLE）的绿色方法，从啤酒糟（BSG）中提取蛋白质并获得高活性提取物。采用 Box-Behnken 实验设计研究了提取参数对蛋白质含量（PC）、总酚含量（TPC）和提取物抗氧化活性的影响。结果与传统碱性超声辅助提取（UAE）的结果进行了比较。PLE 条件的选择使提取物的 PC 和 TPC 可以得到调节。PLE 提取的蛋白质比 UAE 多 36%。PLE 提取物的抗氧化、胆固醇酯酶抑制和 ACE 抑制活性均高于 UAE 提取物。HPLC-MS/MS 观察到提取技术和实验条件对提取蛋白质和释放肽以及酚类化合物的种类和数量有显著影响。在 PLE 提取物中观察到更高比例的疏水性肽，这证明了它们更高的生物活性。
