来自爬行动物的核黄素结合蛋白：与禽类核黄素结合蛋白的比较。

Riboflavin-binding protein from reptiles: a comparison with avian riboflavin-binding proteins.

Abrams V A, McGahan T J, Rohrer J S, Bero A S, White H B

Department of Chemistry, University of Delaware, Newark 19716.

Comp Biochem Physiol B. 1988;90(1):243-7. doi: 10.1016/0305-0491(88)90068-5.

Abstract

Riboflavin-binding protein (RBP) has been isolated for the first time from reptilian sources. 2. RBP from eggs of Python molurus (Indian python) and Chrysemys picta (painted turtle) has been isolated and compared to RBP from Gallus gallus domesticus (chicken), a well-characterized protein, and a newly isolated RBP from Cairina moschata (Muscovy duck). 3. Each of the proteins is phosphorylated and glycosylated. 4. The ratio of riboflavin binding to protein is 1:1 and the KD for each protein is between 1-3 nM. 5. The mol. wts, different for each species, range from 30,000-40,000, with the reptilian proteins being approx. 10,000 larger than the avian proteins.

摘要

核黄素结合蛋白（RBP）首次从爬行动物中分离出来。2. 已从网纹蟒（印度蟒）和锦龟的卵中分离出RBP，并与家鸡（一种特征明确的蛋白质）的RBP以及新分离出的疣鼻栖鸭的RBP进行了比较。3. 每种蛋白质都进行了磷酸化和糖基化修饰。4. 核黄素与蛋白质的结合比例为1:1，每种蛋白质的解离常数在1 - 3 nM之间。5. 每种物种的分子量不同，范围在30,000 - 40,000之间，其中爬行动物的蛋白质比鸟类蛋白质大约大10,000。