纯化的环鸟苷酸依赖性蛋白激酶对不同物种组织中酸溶性染色质蛋白的磷酸化作用。
Phosphorylation of acid-soluble chromatin proteins from tissues of different species by purified cyclic GMP-dependent protein kinase.
作者信息
Linnala-Kankkunen A, Palvimo J, Mäenpää P H
机构信息
Department of Biochemistry, University of Kuopio, Finland.
出版信息
Comp Biochem Physiol B. 1988;90(1):91-4. doi: 10.1016/0305-0491(88)90041-7.
PMID:3396332
Abstract
- Phosphorylation of acid-soluble chromatin proteins from thymus or liver of calf, rabbit, pig, rat, rooster and trout by purified cyclic GMP-dependent protein kinase was studied in vitro using acetic acid-urea slab gel electrophoresis and autoradiography. 2. HMG 14, histone H1 and an unknown band representing probably a proteolytic fragment of histone H1 were phosphorylated in all mammals studied. 3. In avian liver, HMG 14 showed no phosphorylation and histone H1 was replaced by a H1(0)/H5-like heavily phosphorylated protein. 4. The only 32P-acceptor in trout liver apparently belongs to the C/D-family of acid-soluble chromatin proteins. H6-protein was not phosphorylated.
摘要
- 利用醋酸 - 尿素平板凝胶电泳和放射自显影技术，在体外研究了纯化的环鸟苷酸依赖性蛋白激酶对来自小牛、兔子、猪、大鼠、公鸡和鳟鱼胸腺或肝脏的酸溶性染色质蛋白的磷酸化作用。2. 在所有研究的哺乳动物中，高迁移率族蛋白14（HMG 14）、组蛋白H1以及一条可能代表组蛋白H1蛋白水解片段的未知条带都发生了磷酸化。3. 在禽类肝脏中，HMG 14未显示磷酸化，组蛋白H1被一种H1(0)/H5样高度磷酸化的蛋白所取代。4. 鳟鱼肝脏中唯一的32P受体显然属于酸溶性染色质蛋白的C/D家族。H6蛋白未发生磷酸化。
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