[Inhibitory effect of citrus fermentation liquor on liver fibrosis in mice].

OBJECTIVE

To investigate the inhibitory effect of citrus fermentation liquor on liver fibrosis in mice.

OBJECTIVE

Mouse models of liver fibrosis were established by intraperitoneal injection of CCl in 105 male C57BL/6 mice, followed by gavage of 0.1 mL 40% CCl olive oil 3 times a week (model group, =49) or daily gavage of citrus liquor at the dose of 0.26 mL (citrus liquor group, =56) for 8 weeks. Seven mice receiving only olive oil treatment (0.1 mL, 3 times a week) and another 7 treated with citrus liquor served as the control group. Liver tissues and serum samples were collected from 7 mice in the citrus liquor group and model group each week and from the mice in the two control groups at the 8th week for pathological examination of the liver tissues using HE staining and Sirius red staining and for determination of the biochemical indexes of liver function.

OBJECTIVE

The mice in the model group showed progressively worsened liver fibrosis with obvious hepatic steatosis, necrosis and inflammatory cell infiltration. These liver pathologies were much ameliorated in citrus liquor group, which showed significantly reduced vacuolation, inflammatory cell infiltration, collagen deposition and the Ishak score of the liver tissue ( < 0.05). Serum levels of cholyglycine, alanine aminotransferase, transglutaminase and alanine aminotransferase were all significantly lower in citrus liquor group than in the model group ( < 0.05).

OBJECTIVE

citrus fermentation liquor has protective effect on the liver and can significantly ameliorate liver fibrosis in mice.