Friedrich K, Woolley P, Steinhäuser K G
Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
Eur Biophys J. 1988;16(1):45-51. doi: 10.1007/BF00255325.
Using the technique of singlet-singlet (Förster-type) resonance energy transfer, we have determined five distances in the programmed ribosome, either with the P site or with both the A and the P sites occupied. Two of the distances are new and two agree with earlier measurements; the fifth showed disagreement in detail with earlier results of others, but a consistent general trend. The distances substantiate a current model for the location of ribosomally bound tRNA, except in regard of the position of the 3' end of P-site tRNA, which seems according to our results to lie too far away from the 3' terminus of the 16 S RNA to be accommodated in the model. We present new evidence for the hypothesis that anomalously charged tRNA does not bind to the cognately programmed A site in the same way as does tRNA charged with an amino acid. Occupation of the A site restricts mobility of the 3' end of tRNA in the P site.
利用单重态-单重态（福斯特型）共振能量转移技术，我们测定了处于编程状态的核糖体中的五个距离，这些核糖体要么P位点被占据，要么A位点和P位点都被占据。其中两个距离是新测定的，两个与早期测量结果一致；第五个距离在细节上与其他研究人员早期的结果不一致，但总体趋势是一致的。这些距离证实了目前关于核糖体结合tRNA位置的模型，除了P位点tRNA的3'端位置，根据我们的结果，它似乎离16S RNA的3'末端太远，无法纳入该模型。我们为以下假设提供了新的证据：异常带电的tRNA与携带氨基酸的tRNA不同，它不会以相同的方式结合到同源编程的A位点。A位点的占据限制了P位点tRNA 3'端的移动性。
