AMR Laboratory, Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Puttaparthi, India.
Department of Microbiology, Sri Sathya Sai Institute of Higher Medical Sciences, Prasanthigram, India.
J Glob Antimicrob Resist. 2021 Jun;25:323-325. doi: 10.1016/j.jgar.2021.04.004. Epub 2021 May 6.
The genus Burkholderia comprises rod-shaped, non-spore-forming, obligately aerobic Gram-negative bacteria that is found across diverse ecological niches. Burkholderia contaminans, an emerging pathogen associated with cystic fibrosis, is frequently isolated from contaminated medical devices in hospital settings. The aim of this study was to understand the genomic characteristics, antimicrobial resistance profile and virulence determinants of B. contaminans strain SBC01 isolated from the eye of a patient hit by a cow's tail.
A hybrid sequence of isolate SBC01 was generated using Illumina HiSeq and Oxford Nanopore Technology platforms. Unicycler was used to assemble the hybrid genomic sequence. The draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline. Antimicrobial susceptibility testing was performed by VITEK®2. Antimicrobial resistance and virulence genes were identified using validated bioinformatics tools.
The assembled genome size is 8 841 722 bp with a G+C content of 66.33% distributed in 19 contigs. Strain SBC01 was found to possess several antimicrobial resistance and efflux pump genes. The isolate was susceptible to tetracyclines, meropenem and ceftazidime. Many genes encoding potential virulence factors were identified.
Burkholderia contaminans SBC01 belonging to sequence type 482 (ST482) is a multidrug-resistant strain containing diverse antimicrobial resistance genes, revealing the risks associated with infections by new Burkholderia spp. The large G+C-rich genome has a myriad of virulence factors, highlighting its pathogenic potential. Thus, while providing insights into the antimicrobial resistance and virulence potential of this uncommon species, the present analysis will aid in understanding the evolution and speciation in the Burkholderia genus.
伯克霍尔德氏菌属包含杆状、非孢子形成、严格需氧的革兰氏阴性细菌，分布于各种生态环境中。与囊性纤维化相关的新兴病原体伯克霍尔德氏菌污染，经常从医院环境中污染的医疗器械中分离出来。本研究旨在了解从被牛尾巴击中的患者眼部分离出的 B. contaminans 菌株 SBC01 的基因组特征、抗菌药物耐药谱和毒力决定因素。
使用 Illumina HiSeq 和 Oxford Nanopore 技术平台生成了 SBC01 分离株的混合序列。使用 Unicycler 组装混合基因组序列。使用 NCBI Prokaryotic Genome Annotation Pipeline 对草案基因组进行注释。使用 VITEK®2 进行抗菌药物敏感性测试。使用经过验证的生物信息学工具鉴定抗菌药物耐药性和毒力基因。
组装的基因组大小为 8841722 bp，G+C 含量为 66.33%，分布在 19 个 contigs 中。发现 SBC01 菌株携带多种抗菌药物耐药性和外排泵基因。该分离株对四环素类、美罗培南和头孢他啶敏感。鉴定出许多编码潜在毒力因子的基因。
属于序列型 482（ST482）的 B. contaminans SBC01 是一株多药耐药菌株，含有多种抗菌药物耐药基因，揭示了新的伯克霍尔德氏菌属感染相关的风险。富含 G+C 的大型基因组具有多种毒力因子，突出了其致病潜力。因此，本分析不仅为理解该不常见物种的抗菌药物耐药性和毒力潜力提供了依据，还将有助于理解伯克霍尔德氏菌属的进化和物种形成。
