Henningsen Eva C, Omidvar Vahid, Della Coletta Rafael, Michno Jean-Michel, Gilbert Erin, Li Feng, Miller Marisa E, Myers Chad L, Gordon Sean P, Vogel John P, Steffenson Brian J, Kianian Shahryar F, Hirsch Cory D, Figueroa Melania
Department of Plant Pathology, University of Minnesota, St. Paul, MN, United States.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, United States.
Front Plant Sci. 2021 Apr 21;12:657796. doi: 10.3389/fpls.2021.657796. eCollection 2021.
Wheat stem rust disease caused by f. sp. () is a global threat to wheat production. Fast evolving populations of limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with infection in susceptible and resistant genotypes as well as the non-host . We targeted our analysis to genes with differential expression in and genes suppressed or not affected in and report several processes potentially linked to susceptibility to , such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to through removal or modification of putative susceptibility genes.
由禾柄锈菌小麦专化型（Puccinia graminis f. sp. tritici）引起的小麦秆锈病是对小麦生产的全球性威胁。该病原菌快速进化的群体限制了植物遗传抗性的功效，并制约了病害管理策略。了解导致锈菌感染和感病性的分子机制，可为通过感病性的遗传缺失来部署作物抗性提供新策略。我们采用基于比较转录组和直系同源引导的方法，来表征与易感和抗病小麦基因型以及非寄主大麦中禾柄锈菌感染相关的基因表达变化。我们将分析目标锁定在禾柄锈菌中差异表达的基因以及在大麦中被抑制或未受影响的基因，并报告了几个可能与对禾柄锈菌感病性相关的过程，如细胞死亡抑制和光合作用受损。我们通过基因共表达网络分析对方法进行补充，以识别通过去除或修饰假定的感病基因来赋予小麦对禾柄锈菌抗性的靶点。
