Otero-Colina Gabriel, Ramos-González Pedro Luis, Chabi-Jesus Camila, Freitas-Astúa Juliana, Tassi Aline D, Kitajima Elliot W
Campus Montecillo, Colegio de Potsgraduados, 56320 Montecillo, Edo. México México.
Laboratório de Biologia Molecular Aplicada, Instituto Biológico, Av. Conselheiro Rodrigues Alves 1252, São Paulo, SP 04014-902 Brazil.
Virusdisease. 2021 Mar;32(1):167-172. doi: 10.1007/s13337-021-00676-5. Epub 2021 Mar 20.
For the first time, an isolate of the dichorhavirus orchid fleck virus (OFV, family ) was found infecting an orchid plant in Mexico. The infected sample of was collected in a nursery in Lagunillas, municipality of Zihuateutla, Edo. Puebla. Mites gathered on this plant were analyzed by light and scanning electron microscopy, which consistently indicated the presence of adults of the species , the common vector of OFV Viral identification was based on symptoms, cytopathology, and reverse transcriptase-PCR/sequencing of genome fragments of the RNA1 and 2 molecules. Since isolates of OFV causing citrus leprosis have been previously detected in the Mexican states of Chiapas, Querétaro, and Jalisco, we promote a pertinent discussion and thought-provoking questions regarding the epidemiology and putative evolution of OFV.
首次在墨西哥发现一种二分体病毒——兰花斑点病毒（OFV， 科）的分离株感染一种兰花植物。感染样本采自普埃布拉州锡瓦特奥特拉市拉古尼利亚斯的一家苗圃。通过光学显微镜和扫描电子显微镜对聚集在这种植物上的螨虫进行分析，结果一致表明存在该物种的成虫，即OFV的常见传播媒介。病毒鉴定基于症状、细胞病理学以及RNA1和2分子基因组片段的逆转录聚合酶链反应/测序。由于此前已在墨西哥恰帕斯州、克雷塔罗州和哈利斯科州检测到导致柑橘麻风病的OFV分离株，我们针对OFV的流行病学和假定进化展开了相关讨论并提出了发人深省的问题。
