Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui.
作者信息
Harel M, Shoham M, Frolow F, Eisenberg H, Mevarech M, Yonath A, Sussman J L
机构信息
Department of Structural Chemistry, Weizmann Institute of Science, Rehovot, Israel.
出版信息
J Mol Biol. 1988 Apr 5;200(3):609-10. doi: 10.1016/0022-2836(88)90547-5.
PMID:3398050
Abstract
Malate dehydrogenase from the extreme halophile Halobacterium marismortui crystallizes in highly concentrated phosphate solution in space group 12 with cell dimensions a = 113.8 A, b = 122.8 A, c = 126.7 A, beta = 98.1 degrees. The halophilic enzyme was found to be unstable at lower concentrations of phosphate. It associates with unusually large amounts of water and salt, and the combined particle volume shows a tight fit in the unit cell.
摘要
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