Amato D A, Lagakos S W
Harvard University, Boston, MA.
J Natl Cancer Inst. 1988 Aug 17;80(12):919-25. doi: 10.1093/jnci/80.12.919.
The ED01 experiment, which involved determination of the effective dose to produce a 1% tumor rate, was carried out by the National Center for Toxicological Research (NCTR). The study involved greater than 20,000 BALB/c female mice exposed to various doses of 2-acetylaminofluorene, a chemical known to produce bladder and liver carcinomas. After death, tissues from each animal were evaluated for the presence of several types of tumors by one of a team of NCTR pathologists. After the ED01 experiment was completed, the Society of Toxicology commissioned another pathologist to carry out an independent review of the bladder and liver specimens from a stratified sample of the mice. There were substantial differences in the diagnoses of both tumor types by the pathologists, but the implications for detection of a dose-response relationship are important only for liver carcinomas.
国家毒理学研究中心（NCTR）开展了ED01实验，该实验旨在确定产生1%肿瘤发生率的有效剂量。该研究涉及20000多只BALB/c雌性小鼠，它们被暴露于不同剂量的2-乙酰氨基芴（一种已知会引发膀胱癌和肝癌的化学物质）。在小鼠死亡后，国家毒理学研究中心的一组病理学家中的一位对每只动物的组织进行了多种肿瘤类型的评估。ED01实验完成后，毒理学学会委托另一位病理学家对从小鼠分层样本中获取的膀胱和肝脏标本进行独立审查。病理学家对这两种肿瘤类型的诊断存在显著差异，但对于检测剂量反应关系而言，其影响仅对肝癌具有重要意义。
