Appl Opt. 2021 Apr 20;60(12):3365-3373. doi: 10.1364/AO.416943.
The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells. Digital holographic tomography (DHT) and developed data post-processing algorithms were used for quantitative estimation of changes in optical and morphological parameters of cells. We calculated and compared data on the refractive index, thickness, and projected area of 4T1 breast tumor cells in control untreated specimens and those treated with doxorubicin hydrochloride (DOX), canth, and their combinations. Post-treatment changes in cellular morphology recorded by DHT demonstrated that the two drugs led to noticeably different morphological changes in cells that can be presumably associated with different pathways of their death, apoptosis, or necrosis. The effect of combined treatment with these two drugs strongly depended on their relative concentrations and could lead to changes characteristic either for DOX or for canth; however, being more profound than those obtained when using each drug solely. The results obtained by DHT are in a good correspondence with commonly used cell viability analysis and immunofluorescent analysis of changes in cellular cytoskeleton.
本研究聚焦于一种提供非侵入性监测和评估中药斑蝥复合物（canth）对 4T1 乳腺癌细胞抗肿瘤效果的方法。采用数字全息断层扫描（DHT）和开发的数据后处理算法，对细胞的光学和形态参数变化进行定量估计。我们计算并比较了对照组未处理标本和经盐酸多柔比星（DOX）、斑蝥以及它们的组合处理的 4T1 乳腺癌细胞的折射率、厚度和投影面积数据。DHT 记录的细胞形态后处理变化表明，这两种药物导致细胞发生明显不同的形态变化，这可能与它们的死亡途径不同有关，即凋亡或坏死。这两种药物联合治疗的效果强烈依赖于它们的相对浓度，并可能导致与 DOX 或斑蝥各自作用相关的变化；然而，其效果比单独使用每种药物时更为显著。DHT 获得的结果与常用的细胞活力分析和细胞骨架变化的免疫荧光分析结果具有良好的一致性。
