Department of General Surgery, Antalya Training and Research Hospital, University of Health Sciences, Antalya, Turkey.
Department of Biochemistry, Antalya Training and Research Hospital, University of Health Sciences, Antalya, Turkey.
Turk J Med Sci. 2021 Oct;51(5):2716-2726. doi: 10.3906/sag-2101-153. Epub 2021 Oct 21.
BACKGROUND: To evaluate the potential protective effects of boric acid (BA) in experimental cholestatic liver ischemia reperfusion (IR) injury model. METHODS: The study included 24 female rats which were divided into 3 groups each containing 8 rats. The control group (Group 1) only received laparotomy. In the IR group (Group 2) biliary tract ligation was applied and 1 week later 45 min ischemia and 1 h reperfusion with relaparotomy without any treatment was implemented. In the treatment BA+IR group (Group 3). 1 week after the biliary ligation intraperitoneal administration of 200 mg/kg BA was given 10 min before the ischemia for 45 min and reperfusion for 1 h with relaparotomy. Liver tissue and blood samples were taken for histopathological and biochemical examination. Ischemia modified albumin (IMA), SCUBE1, total antioxidant status (TAS), and total oxidant status (TOS) levels were also examined. RESULTS: Compared to control, groups IR and BA+IR had higher serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total, and direct bilirubin levels. Albumin value was high in the control group and low in the other groups. In terms of IMA levels there was no significant difference between groups (p > 0.05). When SCUBE-1 levels were examined groups IR and BA+IR were significantly higher than the group 1. TAS was highest in the group BA+IR whereas TOS was highest in the group IR and lower in the group BA+IR. In histopathological analysis, loss of intercellular border loss in hepatocytes, diffuse nuclear pycnosis and mild to moderate neutrophilic cell infiltration were observed in the IR group. Statistically significant dissociation, hemorrhage and severe neutrophilic cell infiltration were seen in hepatocytes of rats with IR (p < 0.05). DISCUSSION: BA has promising results in the treatment of experimental IR injury of the cholestatic liver because of its antioxidant effects. It may be used in clinical practice after more extensive studies about the effects of BA on IR injury of the cholestatic liver.
背景：评估硼酸（BA）在实验性胆汁淤积性肝缺血再灌注（IR）损伤模型中的潜在保护作用。
方法：本研究纳入 24 只雌性大鼠，随机分为 3 组，每组 8 只。对照组（第 1 组）仅接受剖腹术。在 IR 组（第 2 组），结扎胆管，1 周后进行 45 分钟缺血和 1 小时再灌注，再次剖腹术但不进行任何治疗。在治疗 BA+IR 组（第 3 组），胆管结扎后 1 周，腹腔内给予 200mg/kg BA，缺血前 10 分钟给予 BA，缺血 45 分钟，再灌注 1 小时，再次剖腹术。采集肝组织和血液样本进行组织病理学和生化检查。还检查了缺血修饰白蛋白（IMA）、SCUBE1、总抗氧化状态（TAS）和总氧化状态（TOS）水平。
结果：与对照组相比，IR 组和 BA+IR 组的血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、总胆红素和直接胆红素水平更高。白蛋白值在对照组较高，而在其他组较低。在 IMA 水平方面，各组之间无显著差异（p＞0.05）。当检查 SCUBE-1 水平时，IR 组和 BA+IR 组明显高于第 1 组。BA+IR 组的 TAS 最高，而 IR 组的 TOS 最高，BA+IR 组的 TOS 较低。组织病理学分析显示，IR 组肝细胞的细胞间边界丧失、弥漫性核固缩和轻度至中度中性粒细胞浸润。IR 组大鼠的肝细胞中可见明显分离、出血和严重的中性粒细胞浸润（p＜0.05）。
讨论：由于其抗氧化作用，BA 在治疗实验性胆汁淤积性肝 IR 损伤方面具有良好的效果。在对 BA 对胆汁淤积性肝 IR 损伤的影响进行更广泛的研究后，它可能会在临床实践中使用。
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