用于同时测定多种大鼠脑代谢物水平的体内1H-NMR程序，不受水和脂质信号干扰。

In vivo 1H-NMR procedure to determine several rat cerebral metabolite levels simultaneously, undisturbed by water and lipid signals.

作者信息

De Graaf A A, Bovee W M, Deutz N E, Chamuleau R A

机构信息

Delft University of Technology, Department of Applied Physics, The Netherlands.

出版信息

Magn Reson Imaging. 1988 May-Jun;6(3):255-61. doi: 10.1016/0730-725x(88)90399-2.

DOI:10.1016/0730-725x(88)90399-2

PMID:3398731

Abstract

Methods developed for in vivo 1H-NMR spectroscopy are evaluated and applied using conscious rats. Good quality 1H-spectra of the brain are obtained using a surface coil and a spin echo pulse sequence with the binomial 1-1 and 2-2 water suppression pulses. However, comparing spectra from various rats with each other the water and lipid signals, which cause spectral overlap problems, may differ while the other spectral peaks agree well. Spatially one- and two-dimensional 1H spectroscopic imaging of the rat brain shows that the former signals stem from distinct spatial regions localized close to the rf coil. From a spectroscopic image, a spectrum over a limited spatial region is constructed in which the water signals are strongly reduced, the lipid signals are eliminated and lactic acid can be observed clearly simultaneously with other metabolites.

摘要

利用清醒大鼠对体内1H核磁共振波谱法所开发的方法进行评估和应用。使用表面线圈和具有二项式1-1和2-2水抑制脉冲的自旋回波脉冲序列可获得高质量的脑1H谱。然而，相互比较不同大鼠的谱图时，导致谱峰重叠问题的水和脂质信号可能不同，而其他谱峰则吻合良好。对大鼠脑进行空间一维和二维1H波谱成像显示，前者信号源自靠近射频线圈的不同空间区域。从波谱图像中构建一个有限空间区域上的谱图，其中水信号大幅降低，脂质信号被消除，同时能清晰观察到乳酸以及其他代谢物。