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前列腺素E1触发Nrf2/HO-1信号通路以抵抗氯化血红素诱导的小鼠皮质神经元毒性。

PGE1 triggers Nrf2/HO-1 signal pathway to resist hemin-induced toxicity in mouse cortical neurons.

作者信息

Shen Jiabing, Cao Mao-Sheng, Zhou Tingting, Chen Ying, Liang Jingjing, Song Yan, Xue Chengbin, Cao Mao-Hong, Ke Kaifu

机构信息

Department of Neurology, Affiliated Hospital of Nantong University, Nantong, China.

Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China.

出版信息

Ann Transl Med. 2021 Apr;9(8):634. doi: 10.21037/atm-20-5839.

DOI:10.21037/atm-20-5839
PMID:33987332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8106031/
Abstract

BACKGROUND

Prostaglandin E1 (PGE1) exerts various pharmacological effects such as membrane stabilization, anti-inflammatory functions, vasodilation, and platelet aggregation inhibition. We have previously demonstrated that PGE1 has a beneficial impact on patients suffering from intracerebral hemorrhage (ICH). The related mechanism underlying PGE1's beneficial effect on ICH treatment needs further exploration.

METHODS

The present study elucidates the mechanism of PGE1 on ICH treatment using a neuronal apoptosis model . The mouse primary cortical neurons were pretreated with different concentrations of PGE1, followed by the treatment with hemin, the main catabolite in whole blood, to mimic the clinical ICH.

RESULTS

Comparing with the vehicle-treated group, PGE1 prevented cultured cortical neurons from the accumulation of inhibited intracellular levels of reactive oxygen species (ROS), amelioration of mitochondrial membrane potential, and hemin-induced apoptosis. The reduction of ROS and apoptosis were associated with the up-regulation of Heme oxygenase-1 (HO-1) expression. Knockdown of nuclear transcription factor erythroid 2-related factor (Nrf2) by siRNA attenuated the upregulation of HO-1 as well as the protective effect of PGE1.

CONCLUSIONS

Our work suggests that the Nrf2/HO-1 molecular pathway may play a crucial role in treating ICH patients with PGE1 and may represent novel molecular targets, resulting in discovering new drugs for ICH treatment.

摘要

背景

前列腺素E1（PGE1）具有多种药理作用，如膜稳定、抗炎、血管舒张和抑制血小板聚集。我们之前已经证明PGE1对脑出血（ICH）患者有有益影响。PGE1对ICH治疗产生有益作用的相关机制需要进一步探索。

方法

本研究使用神经元凋亡模型阐明PGE1对ICH治疗的机制。用不同浓度的PGE1预处理小鼠原代皮质神经元，然后用全血中的主要分解代谢产物血红素进行处理，以模拟临床ICH。

结果

与载体处理组相比，PGE1可防止培养的皮质神经元中活性氧（ROS）细胞内水平的积累受到抑制、线粒体膜电位得到改善，并防止血红素诱导的凋亡。ROS的减少和凋亡与血红素加氧酶-1（HO-1）表达的上调有关。用小干扰RNA（siRNA）敲低核转录因子红细胞2相关因子（Nrf2）可减弱HO-1的上调以及PGE1的保护作用。

结论

我们的研究表明，Nrf2/HO-1分子途径可能在PGE1治疗ICH患者中起关键作用，可能代表新的分子靶点，从而为ICH治疗发现新药。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/4370857e240e/atm-09-08-634-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/c6cc9cb86590/atm-09-08-634-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/1ce321bb4411/atm-09-08-634-f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/bebfea0ef3a4/atm-09-08-634-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/4370857e240e/atm-09-08-634-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/c6cc9cb86590/atm-09-08-634-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/1ce321bb4411/atm-09-08-634-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/85d1a14fedff/atm-09-08-634-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/4eca969f9d85/atm-09-08-634-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/bebfea0ef3a4/atm-09-08-634-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39aa/8106031/4370857e240e/atm-09-08-634-f6.jpg

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3
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4
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