Pontificia Universidad Católica del Ecuador, Facultad de Ciencias Exactas y Naturales, Laboratorio de Genética Evolutiva, Avenida 12 de Octubre 1076 y Roca, Quito, Ecuador.
J Insect Sci. 2021 May 1;21(3). doi: 10.1093/jisesa/ieab032.
The guarani group of Drosophila genus (Diptera: Drosophilidae) is formed by 24 species however the relationship of these species is not clear. In the present study are described the karyotypes of Drosophila sachapuyu Peñafiel and Rafael, 2018 and Drosophila zamorana Peñafiel and Rafael, 2018, two Andean species members of the guarani group. Mitotic chromosomes from cerebral ganglia of third stand larval were obtained by thermal shock and cell suspension techniques. The karyotype of D. sachapuyu, presents 2n = 10 (4R, 1V; X = R, Y = R) while D. zamorana exhibits karyotype 2n = 12 (5R, 1V; X = V, Y = R).
果蝇属 Guarani 组(双翅目:果蝇科)由 24 个物种组成,但这些物种之间的关系尚不清楚。本研究描述了安第斯山脉物种果蝇 sachapuyu Peñafiel 和 Rafael, 2018 和 Drosophila zamorana Peñafiel 和 Rafael, 2018 的染色体核型,它们是 Guarani 组的成员。通过热休克和细胞悬浮技术从第三龄幼虫的脑神经获得有丝分裂染色体。D. sachapuyu 的核型为 2n = 10(4R, 1V;X = R, Y = R),而 D. zamorana 的核型为 2n = 12(5R, 1V;X = V, Y = R)。