Department of Environmental Science, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, PR China.
Department of Environmental Science, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, PR China.
J Environ Manage. 2021 Aug 15;292:112750. doi: 10.1016/j.jenvman.2021.112750. Epub 2021 May 12.
Constructed wetlands (CWs) have been proved to be an alternative to the treatment of various wastewater. However, there are few studies focused on the removal performance and mechanisms of pollutants in pilot-scale CWs packed with novel solid carbon. In this study, we investigated the effect of poly-3-hydroxybutyrate-co-3-hydroxyvalerate/polyacetic acid (PHBV/PLA) blends as carbon source on pollutant's transformation, microbial communities and functional genes in pilot-scale aeration-anoxic two-stage CWs for polishing rural runoff in southern China. Results showed a striking improvement of TN removal in CWs with PHBV/PLA blends (64.5%) compared to that in CWs with ceramsite (52.9%). NH-N (61.3-64.6%), COD (40.4-53.8%) and TP (43.6-47.1%) were also removed effectively in both two CWs. In addition, the strains of Rhodocyclaceae and Bacteroidetes were the primary denitrifiers on the surface of PHBV/PLA blends. Further, the aerobic stage induced gathering of 16 S and amoA genes and the anoxic zone with PHBV/PLA blends increased the nirS genes, which fundamentally explained the better denitrification performance in CW based on PHBV/PLA blends. Consequently, this study will provide straightforward guidance for the operation of engineering CWs packed with polymers to govern the low-C/N rural wastewater.
人工湿地（CWs）已被证明是处理各种废水的一种替代方法。然而，很少有研究关注填充新型固体碳的中试规模 CWs 中污染物的去除性能和机制。在本研究中，我们研究了聚 3-羟基丁酸酯-共-3-羟基戊酸/聚乙酸（PHBV/PLA）共混物作为碳源对中国南方农村径流中中试曝气-缺氧两段式 CWs 中污染物转化、微生物群落和功能基因的影响。结果表明，与填陶瓷粒的 CWs（52.9%）相比，CWs 中添加 PHBV/PLA 共混物后 TN 的去除率显著提高（64.5%）。NH-N（61.3-64.6%）、COD（40.4-53.8%）和 TP（43.6-47.1%）也在两种 CWs 中得到有效去除。此外，Rhodocyclaceae 和拟杆菌门的菌株是 PHBV/PLA 共混物表面的主要反硝化菌。此外，好氧阶段诱导了 16S 和 amoA 基因的聚集，而 PHBV/PLA 共混物的缺氧区增加了 nirS 基因，这从根本上解释了基于 PHBV/PLA 共混物的 CW 具有更好的反硝化性能。因此，本研究将为工程 CWs 填充聚合物的运行提供直接指导，以治理低 C/N 农村废水。
