Department of Biological Sciences, University of Medical Sciences, Ondo, Ondo State, Nigeria.
The Laboratory of Diagnosis and Therapy of Infectious Diseases and Cancer, René Rachou Institute, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Minas Gerais, Brazil.
Sci Rep. 2021 May 18;11(1):10530. doi: 10.1038/s41598-021-89929-3.
The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans' sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88-1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10-12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.
本研究旨在确定毛蚴粗抗原（SCA）作为抗曼氏血吸虫抗体检测的诊断靶标。通过超声处理将尾蚴转化为毛蚴，然后匀浆，再离心得到 SCA。使用 ELISA 和斑点印迹免疫分析评估 30 份来自慢性患者的曼氏血吸虫感染血清样本和 30 份非感染人类血清样本中的 SCA。Kato-Katz 或盐水梯度法或两者均用于作为诊断参考。进一步对 Western blot 分析鉴定的 10-12 kDa 免疫反应性带洗脱的蛋白质进行斑点印迹免疫分析。ROC 曲线下的面积为 0.95（AUC 0.95，CI 0.88-1.01，p < 0.0001）。SCA-ELISA 和斑点印迹检测的灵敏度和特异性分别为 96.67%和 86.67%。针对 SCA 的人 IgG 特异性反应在曼氏血吸虫感染个体中明显更高（OD = 0.678 ± 0.249），而非感染人群（OD = 0.235 ± 0.136）（p < 0.0001）。我们的研究表明，SCA 及其 10-12 kDa 成分可用作慢性血吸虫病的诊断工具。
