在杂交罗非鱼中，miR-19a-5p的低表达与二酰基甘油O-酰基转移酶2（DGAT2）的高mRNA表达相关。
Low expression of miR-19a-5p is associated with high mRNA expression of diacylglycerol O-acyltransferase 2 (DGAT2) in hybrid tilapia.
作者信息
Zhong Huan, Zhou Yi, Xu Qian, Yan Jinpeng, Zhang Xiaojin, Zhang Hong, Tang Zhanyang, Xiao Jun, Guo Zhongbao, Luo Yongju, Zhou Luojing
机构信息
College of Animal Science and Technology, Hunan Agriculture University, Changsha, China.
State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, China.
出版信息
Genomics. 2021 Jul;113(4):2392-2399. doi: 10.1016/j.ygeno.2021.05.016. Epub 2021 May 19.
DGAT2 (acyl CoA:diacylglycerol acyltransferase 2) is a key and rate-limiting enzyme that catalyzes the final step of triglyceride (TG) synthesis. In this study, hybrid tilapia were generated from Nile tilapia (♀) and blue tilapia (♂) crossing. The TG content levels in the liver of these tilapia were measured. The results showed that the TG content was higher in the hybrid tilapia. In addition, protein and mRNA expression levels in the tilapia livers were determined. Higher hepatic mRNA and protein expression of DGAT2 in the hybrid fish was found. A luciferase reporter assay with HEK293T cells revealed that miRNA-19a-5p targeted the 3'UTR of DGAT2, suggesting a direct regulatory mechanism. Using qRT-PCR, we found that DGAT2 mRNA levels had a negative correlation with miRNA-19a-5p expression in Nile tilapia and hybrid. Taken together, these findings provide evidence that miRNA-19a-5p is involved in TG synthesis in the regulation of lipid metabolism in tilapia.
二酰甘油酰基转移酶2（酰基辅酶A：二酰甘油酰基转移酶2）是催化甘油三酯（TG）合成最后一步的关键限速酶。在本研究中，尼罗罗非鱼（♀）和蓝罗非鱼（♂）杂交产生了杂交罗非鱼。测定了这些罗非鱼肝脏中的TG含量水平。结果表明，杂交罗非鱼的TG含量更高。此外，还测定了罗非鱼肝脏中的蛋白质和mRNA表达水平。发现杂交鱼肝脏中DGAT2的mRNA和蛋白质表达更高。用HEK293T细胞进行的荧光素酶报告基因检测显示，miRNA-19a-5p靶向DGAT2的3'UTR，提示存在直接调控机制。通过qRT-PCR，我们发现尼罗罗非鱼和杂交罗非鱼中DGAT2 mRNA水平与miRNA-19a-5p表达呈负相关。综上所述，这些发现提供了证据表明miRNA-19a-5p参与罗非鱼脂质代谢调控中的TG合成。
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