Komatsu Tetsuya, Yoshida Erina, Shigenaga Ayumi, Yasuie Nozomi, Uchiyama Shintaro, Takamura Yuji, Sugie Kennosuke, Kimura Kumiko, Haritani Makoto, Shibahara Tomoyuki
Aichi Prefectural Chuo Livestock Hygiene Service Center, 1-306 Jizono, Miaicho, Okazaki, Aichi 444-0805, Japan.
Miyazaki Prefectural Livestock Hygiene Service Center, 3151-1 Shimonaka, Sadowaracho, Miyazaki, Miyazaki 880-0212, Japan.
J Vet Med Sci. 2021 Jul 13;83(7):1113-1119. doi: 10.1292/jvms.21-0166. Epub 2021 May 24.
One calf died (No. 1) and another was euthanized following astasia (No. 2). Histopathological examination revealed suppurative meningoencephalitis in these calves. Klebsiella pneumoniae antigens were detected in lesions. Thymocytes were decreased in the thymus cortex in both cases. 16S rRNA gene sequencing of the No. 1 isolate and bacterial extracts from formalin fixed paraffin embedded sections of No. 2 revealed that both samples were K. pneumoniae. The No. 1 isolate showed multidrug resistance against penicillin antibiotics, fosfomycin, streptomycin, macrolide antibiotics, tetracycline antibiotics, and clindamycin. Immunosuppression is a significant septicemic K. pneumoniae infection risk factor. Our study provides new aspects regarding K. pneumoniae infections in cattle, bacterial meningoencephalitis differentiation, and K. pneumoniae and bacterial meningoencephalitis treatments.
一头小牛死亡（编号1），另一头在出现站立不能后实施安乐死（编号2）。组织病理学检查显示这些小牛患有化脓性脑膜脑炎。在病变部位检测到肺炎克雷伯菌抗原。两例小牛的胸腺皮质中胸腺细胞均减少。对编号1的分离株以及编号2经福尔马林固定石蜡包埋切片的细菌提取物进行16S rRNA基因测序，结果显示两个样本均为肺炎克雷伯菌。编号1的分离株对青霉素类抗生素、磷霉素、链霉素、大环内酯类抗生素、四环素类抗生素和克林霉素表现出多重耐药性。免疫抑制是肺炎克雷伯菌败血症感染的一个重要风险因素。我们的研究为牛肺炎克雷伯菌感染、细菌性脑膜脑炎鉴别以及肺炎克雷伯菌和细菌性脑膜脑炎的治疗提供了新的视角。
