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酱香型白酒初始发酵过程中微生物群落演替及其环境驱动因素

Microbial Community Succession and Its Environment Driving Factors During Initial Fermentation of Maotai-Flavor Baijiu.

作者信息

Hao Fei, Tan Yuwei, Lv Xibin, Chen Liangqiang, Yang Fan, Wang Heyu, Du Hai, Wang Li, Xu Yan

机构信息

Kweichow Moutai Distillery Co., Ltd., Guizhou, China.

Key Laboratory of Industrial Biotechnology, Center for Brewing Science and Enzyme Technology, Ministry of Education, Jiangnan University, Wuxi, China.

出版信息

Front Microbiol. 2021 May 6;12:669201. doi: 10.3389/fmicb.2021.669201. eCollection 2021.

Abstract

The microbial composition and environmental factors can take a great influence on community succession during the solid-state fermentation (SSF) of Maotai-flavor Baijiu. In this paper, high-throughput sequencing was used to reveal the dominant microorganisms and the evolution process of microbial community structure in the initial fermentation of Maotai-flavor Baijiu. The correlation analysis was carried out for the relationship between physicochemical factors and fermented microbes. The results showed that microorganisms were obviously enriched and the diversity of bacteria and fungi showed a downward trend during the heap fermentation process of Maotai-flavor Baijiu. However, the diversity of fungi in the pit fermentation process increased. Generally, , , and were the dominant microorganisms in the initial fermentation of Maotai-flavor Baijiu. According to the redundancy analysis, we found that reducing sugar was the key driving factor for microbial succession in the heap fermentation, while acidity, alcohol, and temperature were the main driving forces in pit fermentation. This study revealed the microbial succession and its related environmental factors in the initial fermentation of Maotai-flavor Baijiu, which will enrich our knowledge of the mechanism of solid-state liquor fermentation.

摘要

微生物组成和环境因素对酱香型白酒固态发酵(SSF)过程中的群落演替有很大影响。本文采用高通量测序技术揭示酱香型白酒初始发酵过程中的优势微生物及微生物群落结构的演变过程。对理化因素与发酵微生物之间的关系进行了相关性分析。结果表明,在酱香型白酒堆积发酵过程中微生物明显富集,细菌和真菌的多样性呈下降趋势。然而,窖池发酵过程中真菌的多样性增加。一般来说,芽孢杆菌属、乳杆菌属和曲霉属是酱香型白酒初始发酵中的优势微生物。通过冗余分析发现,还原糖是堆积发酵中微生物演替的关键驱动因素,而酸度、酒精度和温度是窖池发酵中的主要驱动力。本研究揭示了酱香型白酒初始发酵过程中的微生物演替及其相关环境因素,这将丰富我们对固态白酒发酵机制的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4981/8139626/16186e3efa4b/fmicb-12-669201-g001.jpg

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