Laboratório de Entomologia Médica e Forense, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil, 4365, Manguinhos, Cep, Rio de Janeiro 21040-360, Brazil.
Laboratório de Microbiologia do Departamento de Saneamento e Saúde Ambiental, Escola Nacional de Saúde Pública Sérgio Arouca, R. Leopoldo Bulhões, 1480 - Manguinhos, Rio de Janeiro 21041-210, Brazil.
Acta Trop. 2021 Aug;220:105962. doi: 10.1016/j.actatropica.2021.105962. Epub 2021 May 21.
Antimicrobial-resistant bacteria were isolated from muscoid dipterans collected at five different areas of Rio de Janeiro city, in proximity to hospitals. Extracts obtained by maceration of flies were diluted and used as inocula for different culture media, with or without antibiotic (ceftriaxone 1 mg/L) supplementation. Purified isolates were submitted to antimicrobial susceptibility testing (AST). Bacterial identification was performed by MALDI TOF Microflex LT (Bruker Daltonics). A total of 197 bacterial strains were obtained from 117 dipterous muscoids. Forty-two flies (35.9%) carried bacteria resistant to at least one antimicrobial, while 7 insects (5.9%) carried multidrug-resistant bacteria (MDR), which were all members of the family Enterobacteriaceae. Among 10 MDR bacteria (5%), 5 strains (2,5%) were positive by PCR for one or more of the following antibiotic resistance genes: aac(6')-Ib, bla, bla, bla and bla. Analysis of variance (ANOVA) and cluster analysis compared the number of resistant isolates per collection point and showed that a single location was statistically different from the others with regard to resistance. Although there are still no criteria to determine the environmental contamination by resistant bacteria the fact that they have been isolated from flies is an indication of a disseminated contamination. As such, these insects may be useful in monitoring programs of antibiotic resistance in non-hospital environments, where they could function as sentinels.
从里约热内卢市五个不同地区靠近医院的虻蝇中分离出了耐抗生素的细菌。通过浸渍苍蝇获得的提取物被稀释，并用作不同培养基的接种物，有或没有抗生素（头孢曲松 1mg/L）的补充。纯化的分离物进行了抗菌药敏试验（AST）。细菌鉴定通过 MALDI TOF Microflex LT（布鲁克·道尔顿）进行。从 117 只虻蝇中获得了 197 株细菌。42 只苍蝇（35.9%）携带至少一种抗微生物药物的耐药菌，而 7 只昆虫（5.9%）携带多药耐药菌（MDR），均属于肠杆菌科。在 10 株 MDR 细菌（5%）中，5 株（2.5%）通过 PCR 对以下一种或多种抗生素耐药基因呈阳性：aac(6')-Ib、bla、bla、bla 和 bla。方差分析（ANOVA）和聚类分析比较了每个采集点的耐药分离株数量，表明单个采集点在耐药性方面与其他采集点有统计学差异。尽管目前还没有确定环境中耐抗生素细菌污染的标准，但从苍蝇中分离出这些细菌表明存在广泛的污染。因此，这些昆虫可能有助于监测非医院环境中的抗生素耐药性，它们可以作为哨兵。
