白细胞介素-34 及其受体激活物核因子-kB 配体和骨保护素水平在牙周病中的新作用。
Emerging roles of Interleukin-34 together with receptor activator of nuclear factor-kB ligand and osteoprotegerin levels in periodontal disease.
机构信息
Department of Periodontology, Faculty of Dentistry, Ankara Yıldırım Beyazıt University, Ankara, Turkey.
Department of Periodontology, Faculty of Dentistry, Ordu University, Ordu, Turkey.
出版信息
Cytokine. 2021 Aug;144:155584. doi: 10.1016/j.cyto.2021.155584. Epub 2021 May 24.
OBJECTIVE
The dependence between gingival crevicular fluid (GCF) of Interleukin-34 (IL-34) level and Receptor activator of nuclear factor -kB ligand/ osteoprotegerin (RANKL/OPG) ratio in the severity of periodontitis might reveal an unknown pathway of diseases with bone destruction. There is no study about the evaluation of IL-34 levels together with GCF RANKL and OPG levels in periodontitis patients before and after non-surgical periodontal treatment (NSPT). The objectives of this research were to investigate changes in the levels and relative ratios of IL-34, OPG, and RANKL in the GCF of patients with periodontitis before and after NSPT.
MATERIALS AND METHODS
20 healthy participants (CTRL), 20 patients with stage 3-grade B periodontitis and 20 with stage 3-grade C periodontitis were recruited. GCF and clinical periodontal recordings were investigated at the baseline and 6 weeks after NSPT. Enzyme-linked immunosorbent assay (ELISA) were used for quantifying of GCF IL-34, RANKL and OPG levels and their relative ratios were calculated.
RESULTS
Greater values for GCF IL-34 and RANKL levels were found in the both of periodontitis groups than in CTRL group at baseline, whereas GCF OPG levels were statistically lower at baseline (P < 0.05). GCF IL-34 and RANKL levels decreased in the 6th week after NSPT in the both periodontitis groups, while the concentration OPG levels statistically increased (P < 0.05). Significantly positive correlations among the IL-34 with RANKL, sampled-site clinical attachment level (CAL), and gingival index (GI), whereas negative correlation with OPG were reported (P < 0.05).
CONCLUSIONS
GCF IL-34 levels was high in patients with periodontitis and decreased after NSPT and its levels showed positive correlations with RANKL/OPG ratio levels CAL and GI. Determining of IL-34 levels together with RANKL/OPG ratio in GCF may therefore be valuable in detecting high risk individuals with periodontitis patients.
目的
牙龈沟液（GCF）中白细胞介素-34（IL-34）水平与核因子受体激活剂配体/骨保护素（RANKL/OPG）比值在牙周炎严重程度之间的关系可能揭示了一种具有骨破坏的未知疾病途径。在非手术牙周治疗（NSPT）前后，评估牙周炎患者 GCF 中 IL-34 水平以及 GCF 中 RANKL 和 OPG 水平的研究尚不多见。本研究旨在探讨 NSPT 前后牙周炎患者 GCF 中 IL-34、OPG 和 RANKL 水平及其相对比值的变化。
材料和方法
招募了 20 名健康参与者（CTRL）、20 名牙周炎 3 级 B 期患者和 20 名牙周炎 3 级 C 期患者。在基线和 NSPT 后 6 周时进行 GCF 和临床牙周记录检查。采用酶联免疫吸附试验（ELISA）检测 GCF 中 IL-34、RANKL 和 OPG 水平，并计算其相对比值。
结果
在基线时，与 CTRL 组相比，牙周炎两组的 GCF IL-34 和 RANKL 水平均较高，而 GCF OPG 水平则较低（P<0.05）。在 NSPT 后第 6 周，两组牙周炎患者的 GCF IL-34 和 RANKL 水平均降低，而 OPG 浓度则统计学升高（P<0.05）。IL-34 与 RANKL、采样部位临床附着水平（CAL）和牙龈指数（GI）呈显著正相关，与 OPG 呈负相关（P<0.05）。
结论
牙周炎患者 GCF IL-34 水平较高，NSPT 后降低，其水平与 RANKL/OPG 比值、CAL 和 GI 呈正相关。因此，同时测定 GCF 中的 IL-34 水平和 RANKL/OPG 比值可能有助于检测牙周炎高危人群。
Zotero 插件