Bhattacharya J
Department of Physiology, Columbia College of Physicians and Surgeons, New York, New York.
J Appl Physiol (1985). 1988 Jun;64(6):2562-7. doi: 10.1152/jappl.1988.64.6.2562.
The split-drop method has been used to determine filtration rate per unit surface area in the single pulmonary venule. In isolated perfused lungs of nine dogs, blood flow was stopped at different vascular pressures. By means of a double-micropuncture technique under stereomicroscopy, an oil drop was injected in a subpleural venule. The oil drop was then split with a solution of albumin (5.6 g/100 ml) in Ringer lactate. As the Ringer-albumin solution filtered, the distance between the menisci of the split oil drop (split-drop length) decreased. The split-drop geometry and the rate of change of split-drop length were recorded. The calculated venular filtration rate per unit surface area related linearly with vascular pressure (P less than 0.05). The slope of the line equaled venular hydraulic conductivity, which averaged 2.9 +/- 0.02 x 10(-7) ml/(cm2.s.cmH2O). Hydraulic conductivity is lower in lung than in systemic venules.
裂滴法已被用于测定单个肺小静脉单位表面积的滤过率。在9只犬的离体灌注肺中，在不同血管压力下阻断血流。通过立体显微镜下的双微穿刺技术，将一滴油注入胸膜下小静脉。然后用乳酸林格液中含白蛋白（5.6 g/100 ml）的溶液将油滴分开。随着林格-白蛋白溶液滤过，分开的油滴弯月面之间的距离（裂滴长度）减小。记录裂滴的几何形状和裂滴长度的变化率。计算得出的单位表面积小静脉滤过率与血管压力呈线性相关（P<0.05）。该直线的斜率等于小静脉水力传导率，其平均值为2.9±0.02×10⁻⁷ ml/(cm²·s·cmH₂O)。肺中的水力传导率低于体循环小静脉。
