Department of Anesthesiology, University Hospital Duesseldorf, Duesseldorf, Germany.
Methods Mol Biol. 2021;2321:9-15. doi: 10.1007/978-1-0716-1488-4_2.
Colon ascendens stent peritonitis (CASP) is one of the well-established models of experimental abdominal sepsis. In CASP surgery, an open link between the gut lumen and the abdominal cavity is created by placing a stent into the colon ascendens. This mimics well the insufficient intestinal anastomosis. It causes a continuous leakage of the gut contents into the peritoneum and leads therefore to peritonitis and sepsis. The abdominal cavity is opened under general anesthesia and a plastic stent is located through and sutured to the colonic wall. The septic severity in CASP models can be titrated by altering the size of the stent catheter. Therefore, CASP models with small stents sizes are suitable for long-term studies and studies with mild/moderate sepsis severity. Within 24 h, animals develop clinical signs of sepsis. Monitoring of the clinical state, sufficient analgesia, appropriate antibiotics and fluid resuscitation should be performed postoperatively.
升结肠支架腹膜炎（CASP）是一种成熟的实验性腹腔感染模型。在 CASP 手术中，通过将支架放入升结肠来建立肠道腔与腹腔之间的开放联系。这很好地模拟了不完全的肠吻合术。它会导致肠道内容物持续漏入腹膜腔，从而导致腹膜炎和败血症。在全身麻醉下打开腹腔，并将塑料支架穿过并缝合到结肠壁上。通过改变支架导管的大小可以调整 CASP 模型中的感染严重程度。因此，小支架尺寸的 CASP 模型适合长期研究和轻度/中度感染严重程度的研究。在 24 小时内，动物会出现败血症的临床症状。术后应监测临床状态、充分镇痛、适当使用抗生素和液体复苏。
