从粪便中快速制备用于 PCR 鉴定的胃肠道线虫卵。
Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification.
机构信息
Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia.
Gretals Australia, Unit 8/1140 Nepean Highway, Mornington, VIC 3931, Australia.
出版信息
J Microbiol Methods. 2021 Aug;187:106257. doi: 10.1016/j.mimet.2021.106257. Epub 2021 May 29.
Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.
检测胃肠道线虫（GIN）既需要定性检测，也需要定量检测，这是非常理想的。多重和 qPCR 等方法能够提供这样的结果，但可能既繁琐又昂贵。本文提出了一种从粪便样本中快速、低成本制备 GIN 卵的方法，该方法产生的 DNA 适用于 PCR 分析。我们还描述了一套适用于单管多重 PCR 的引物。
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