Tran Vuvi G, Nguyen Na N Z, Kwon Byungsuk
School of Biological Science, University of Ulsan, Ulsan 44610, Korea.
J Fungi (Basel). 2021 May 14;7(5):382. doi: 10.3390/jof7050382.
Invasive fungal infections by frequently cause mortality in immunocompromised patients. Neutrophils are particularly important for fungal clearance during systemic infection, yet little has been known regarding which surface receptor controls neutrophils' antifungal activities. CD137, which is encoded by , belongs to the tumor necrosis receptor superfamily and has been shown to regulate neutrophils in Gram-positive bacterial infection. Here, we used genetic and immunological tools to probe the involvement of neutrophil CD137 signaling in innate defense mechanisms against systemic infection. We first found that mice were susceptible to infection, whereas injection of anti-CD137 agonistic antibody protected the host from infection, suggesting that CD137 signaling is indispensable for innate immunity against infection. Priming of isolated neutrophils with anti-CD137 antibody promoted their phagocytic and fungicidal activities through phospholipase C. In addition, injection of anti-CD137 antibody significantly augmented restriction of fungal growth in mice that received wild-type (WT) neutrophils. In conclusion, our results demonstrate that CD137 signaling contributes to defense mechanisms against systemic infection by promoting rapid fungal clearance.
侵袭性真菌感染经常导致免疫功能低下患者死亡。在系统性感染期间，中性粒细胞对于清除真菌尤为重要，但关于哪种表面受体控制中性粒细胞的抗真菌活性，人们所知甚少。CD137由[具体基因名称]编码，属于肿瘤坏死因子受体超家族，并且已证实在革兰氏阳性细菌感染中可调节中性粒细胞。在此，我们使用遗传学和免疫学工具来探究中性粒细胞CD137信号传导在针对系统性[真菌名称]感染的固有防御机制中的作用。我们首先发现[基因敲除]小鼠易受[真菌名称]感染，而注射抗CD137激动性抗体可保护宿主免受感染，这表明CD137信号传导对于抵抗[真菌名称]感染的固有免疫是不可或缺的。用抗CD137抗体预处理分离的中性粒细胞可通过磷脂酶C促进其吞噬和杀菌活性。此外，注射抗CD137抗体显著增强了接受野生型（WT）中性粒细胞的[小鼠品系]小鼠对真菌生长的限制。总之，我们的结果表明，CD137信号传导通过促进快速清除真菌而有助于抵抗系统性[真菌名称]感染的防御机制。
