Middleton J F, Hunt S, Oates K
Department of Biological Sciences, University of Lancaster, United Kingdom.
Cell Tissue Res. 1988 Aug;253(2):469-75. doi: 10.1007/BF00222305.
Blocks of articular cartilage were taken from tibiae of young adult (8 week) and aged adult (50-60 week) rats; xiphisternal cartilage was obtained from young adult rats. Specimens were quench-frozen in nitrogen slush, freeze-fractured and examined by low-temperature scanning electron microscopy. The results of X-ray microanalysis of frozen-hydrated bulk cartilage are semi-quantitative. The composition of chondrocyte nuclei and cytoplasm are only marginally different. Xiphisternal chondrocytes contain lipid inclusions which show an absence of element peaks and are designated as being neutral lipid. Intra- and extracellular Na, P, S, Cl, K and Ca count rates are significantly different. Cartilage from older rats contains more S and Ca, and less K and Cl in the intercellular matrix than that from young rats. Intracellular K levels are lower in aged than in young rats. The intercellular matrix of xiphisternal cartilage contains larger amounts of S, Na and K, and a smaller amount of Cl compared to that of tibial articular cartilage.
从年轻成年（8周）和老年成年（50 - 60周）大鼠的胫骨获取关节软骨块；剑胸骨软骨取自年轻成年大鼠。标本在液氮中骤冷冷冻、冷冻断裂，并通过低温扫描电子显微镜检查。冷冻水合整块软骨的X射线微分析结果是半定量的。软骨细胞核和细胞质的组成仅有微小差异。剑胸骨软骨细胞含有脂质包涵体，这些包涵体没有元素峰，被认定为中性脂质。细胞内和细胞外的钠、磷、硫、氯、钾和钙计数率有显著差异。与年轻大鼠的软骨相比，老年大鼠的软骨在细胞间基质中含有更多的硫和钙，而钾和氯较少。老年大鼠细胞内的钾水平低于年轻大鼠。与胫骨关节软骨相比，剑胸骨软骨的细胞间基质含有更多的硫、钠和钾，以及更少的氯。
