巴西亚马逊地区的水牛头颈部结核分枝杆菌（Mycobacterium bovis）和其他分枝杆菌感染。

Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon.

机构信息

Post-graduate Program in Animal Science, Federal University of Pará, Belém, Brazil.

Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Brazil.

出版信息

J Infect Dev Ctries. 2021 May 31;15(5):736-741. doi: 10.3855/jidc.13558.

DOI:10.3855/jidc.13558

PMID:34106899

Abstract

INTRODUCTION

Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region.

METHODOLOGY

Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively.

RESULTS

PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.

CONCLUSIONS

The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.

摘要

简介

人畜共患结核病是一种对全球公共卫生具有重要意义的疾病，尤其是在发展中国家。本研究旨在调查牛分枝杆菌和其他分枝杆菌作为巴西亚马逊地区水牛结核病（TB）病因的作用。

方法

在屠宰时的卫生检查中，从 109 头水牛（n=109）的肉芽肿性病变中获得疑似结核病的病变，进行组织病理学评估、分枝杆菌抗原的免疫组织化学（IHC）检测以及 hsp65、IS6110 和 RD4 基因的分子检测（PCR），这些基因分别针对分枝杆菌属、结核分枝杆菌复合体（MTBC）和牛分枝杆菌。

结果

PCR 结果表明 87.2%的病例存在分枝杆菌感染，其中 69.5%为牛分枝杆菌阳性，27.4%属于 MTBC，3.1%可能是非结核分枝杆菌。属特异性分子技术与组织病理学分析之间具有良好的一致性。非牛分枝杆菌引起的结核病病例如此高的频率表明，巴西亚马逊地区与水牛结核病相关的分枝杆菌存在多样化的情况。