Institute for Plant Protection, Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Neustadt an der Weinstrasse 67435, Germany.
Faculty of Biology, Division of Phytopathology, Technische Universität Kaiserslautern, Kaiserslautern 67653, Germany.
Plant Dis. 2021 Dec;105(12):4132-4137. doi: 10.1094/PDIS-12-20-2716-RE. Epub 2021 Nov 30.
The ectoparasitic nematode transmits grapevine fanleaf virus (GFLV) during feeding on grapevine roots, causing fanleaf degeneration in the plant. Hence, resistance breeding is a key to develop novel rootstocks to overcome such threats. In past years, various grapevine species were screened, and a few candidates with partial resistance were identified. However, they were hardly sufficient for viticulture because of their many agronomical defects. To develop reliably resistant rootstocks applicable in viticulture, multiple spp. genotypes were analyzed using root inoculation with nematodes in glass vials as an early and easy evaluation test. Resistance levels were evaluated 35 days after inoculation based on nematode reproduction factors, focusing on juveniles and eggs. Infection of grapevines with GFLV was analyzed after inoculation with viruliferous . With this fast screening system, putative candidates with resistances against have been identified for future breeding programs. Particularly, genotypes with the genetic background of and were found to be nematode-resistant.
食外寄生线虫在取食葡萄根系时传播葡萄扇叶病毒(GFLV),导致植株扇叶退化。因此,抗性育种是开发新砧木以克服此类威胁的关键。过去几年,对各种葡萄品种进行了筛选,鉴定出了少数具有部分抗性的候选品种。然而,由于它们存在许多农艺缺陷,几乎不足以用于葡萄栽培。为了开发可靠的、适用于葡萄栽培的抗性砧木,使用线虫在玻璃小瓶中根接种对多个 spp.基因型进行了分析,作为早期和简单的评估测试。接种后 35 天根据线虫繁殖因子(重点是幼体和卵)评估抗性水平。接种毒力的 后,分析了葡萄感染 GFLV 的情况。利用这种快速筛选系统,已经确定了具有抗线虫特性的候选品种,用于未来的育种计划。特别是,具有 和 遗传背景的基因型被发现具有抗线虫性。