Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Food Funct. 2021 Aug 2;12(15):6950-6963. doi: 10.1039/d1fo00608h.
The aim of this study was to evaluate the physicochemical properties and aroma differences of yellow pea flours fermented by five lactic acid bacteria (LAB) strains including two Lactiplantibacillus, two Lactobacillus, and one Lacticaseibacillus with different fermentation time. The cell population and the pH of pea flour slurry, as well as the proximate chemical composition, amino acids, thermal and pasting properties, surface morphology, and aromatic differences of fermented flours were characterized. The cell population of all strains except for Lactobacillus helveticus was observed to reach above 107 CFU mL-1 after 24 h of fermentation. The fermentation with Lactobacilli resulted in the increase of amino acids and ash contents, and the reduction of fat content. Rapid viscosity analysis indicated that short time (18 h) fermentation with L. helveticus drastically improved the pasting properties of the flours by facilitating starch granule expansion. The aromatic compounds of the fermented yellow pea flours were highly reliant on the strains and fermentation time. The untargeted metabolomics analysis with the aid of multivariate data analysis can discriminate the aroma differences among the fermented yellow pea flours. L. acidophilus fermentation led to the production of three aromatic compounds which may contribute to an improved aromatic profile.
本研究旨在评估五种乳酸菌(LAB)菌株发酵黄豌豆粉的理化特性和香气差异,这五种菌株包括两种植物乳杆菌、两种乳杆菌和一种乳酸片球菌,发酵时间不同。研究了豌豆粉浆的细胞群体和 pH 值、近似化学成分、氨基酸、热特性和糊化特性、表面形态以及发酵粉的香气差异。除了瑞士乳杆菌外,所有菌株的细胞群体在发酵 24 小时后均达到 107 CFU mL-1 以上。乳酸菌发酵导致氨基酸和灰分含量增加,脂肪含量减少。快速粘度分析表明,瑞士乳杆菌在 18 小时的短时间发酵可通过促进淀粉颗粒膨胀,极大地改善面粉的糊化特性。发酵黄豌豆粉的芳香化合物高度依赖于菌株和发酵时间。借助多元数据分析的非靶向代谢组学分析可以区分发酵黄豌豆粉的香气差异。嗜酸乳杆菌发酵产生了三种芳香化合物,可能有助于改善芳香特性。