Labbé, 1896 (Alveolata: Apicomplexa) in frogs: Description of a new species and proposing a new subfamily to accommodate these enigmatic parasites.

Labbé ,1896 (Alveolata: Apicomplexa) is a monotypic genus of renal coccidia found in anurans, particularly in the edible frog kl. (Amphibia: Anura: Ranidae), distributed in different parts of Europe. Here we propose a new species from the Tokyo daruma pond frog, . The coccidium detected in the renal tissue of shared some morphological characteristics with the type species, (Labbé, 1894), reported from kl. . However, in addition to size differences in several oocyst and sporocyst features between these parasites, phylogenetic analysis of gene fragments from two nuclear ribosomal loci and the mitochondrial cytochrome oxidase subunit 1, exposed distinct genetic differences between and our new species. Although our analysis validated the monophyly of with some members of the Toxoplasmatinae Biocca, 1957, Cystoisosporinae Frenkel et al., 1987, and Eumonosporinae Chou et al., 2021 (Sarcocystidae Poche, 1913), comparison of genetic differences between species from and revealed the presence of a greater number of polymorphisms than that observed when comparing inter-species ( spp., spp.) or inter-genus ( vs vs. and vs) variabilities among members of the Sarcocystidae. This indicates that , as re-erected and defined by Modrý et al. (2001, Int. J. Syst. Evol. Microbiol. 51, 767-772), with its homoxenous life cycle, requires placement in its own subfamily. Thus, we propose a new subfamily, Hyaloklossiinae n. subfam., to accommodate two species, from Europe and n. sp. which we describe here from in Japan.