Suppr超能文献

评价五齿萼虾脊兰提取物对白色念珠菌及其毒力因子的抗真菌光动力活性。

Evaluation of the antifungal photodynamic activity of Thymophylla pentachaeta extracts against Candida albicans and its virulence factors.

机构信息

Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.

Herbario, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, 5700 San Luis, Argentina.

出版信息

Phytomedicine. 2021 Sep;90:153608. doi: 10.1016/j.phymed.2021.153608. Epub 2021 May 29.

Abstract

BACKGROUND

Candida albicans is one of the most common causative of opportunistic infections. Treatment of candidiasis is challenging considering the few antifungal drugs available and the increase in resistance. Antimicrobial photodynamic therapy (aPDT) is a recently developed therapeutic option that combines a non-toxic photosensitizer (PS) and light to kill the microbial pathogens. Targeting virulence, defined as the ability of a pathogen to cause overt disease, represents another attractive target for the development of novel antifungal agents. Thymophylla pentachaeta (DC.) Small var. belenidium (DC.) is an endemic plant from Argentina in which the presence of thiophenes, biologically active compounds whose antifungal activity is enhanced by irradiation with Ultraviolet A (UVA), have been already described.

PURPOSE

The purpose of this study was to evaluate the photodynamic antifungal activity of hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts from T. pentachaeta var. belenidium and their inhibitory effects on C. albicans virulence factors as well as biofilm formation and eradication.

STUDY DESIGN/METHODS: Antifungal photodynamic activity of Hex, DCM, EtOAc and MeOH extracts from different parts of the plant were assessed with the microbroth dilution, bioautography and the time-kill assays, under light and darkness conditions. The capacities of the most active extracts of inhibiting Candida virulence factors (adherence to epithelial cells, germ tube and pseudomycelium formation and hydrolytic enzyme secretion) were assessed. In addition, the activity against biofilm formation and eradication has been investigated by reaction with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) that quantifies living cells in these structures.

RESULTS

Hex and DCM extracts from T. pentachaeta roots exhibited high photodynamic antifungal activity against C. albicans [Minimal fungicide concentrations (MFCs)= 7.8 µg/ml] under UVA light irradiation. Chemical analysis of active extracts (Hex and DCM from roots) revealed the presence of photoactive thiophenes. Both extracts generate reactive oxygen species through type I and II mechanisms. These extracts, at sub-inhibitory concentrations, under light conditions decreased the adherence of C. albicans to Buccal Epithelial Cells (BEC), inhibited germ tube formation and reduced esterase production. Finally, they demonstrated activity against preformed biofilms submitted to irradiation (MFCs= 3.91 µg/ml and 15.63 µg/ml for Hex and DCM extracts, respectively).

CONCLUSION

Taking together, results demonstrated the strong photodynamic effects of T. pentachaeta root extracts under UVA irradiation, making them valuable alternatives to the already established antifungal drugs against C. albicans.

摘要

背景

白色念珠菌是最常见的机会性感染病原体之一。由于现有的抗真菌药物有限,而且耐药性不断增加,治疗念珠菌病具有挑战性。光动力抗菌疗法(aPDT)是一种最近开发的治疗选择,它结合了无毒的光敏剂(PS)和光来杀死微生物病原体。靶向毒力,即病原体引起明显疾病的能力,代表了开发新型抗真菌药物的另一个有吸引力的目标。Thymophylla pentachaeta(DC.)Small var. belenidium(DC.)是一种来自阿根廷的特有植物,其中已经描述了噻吩的存在,噻吩是一类具有生物活性的化合物,其抗真菌活性在紫外线 A(UVA)照射下增强。

目的

本研究旨在评估来自 Thymophylla pentachaeta var. belenidium 的正己烷(Hex)、二氯甲烷(DCM)、乙酸乙酯(EtOAc)和甲醇(MeOH)提取物的光动力抗真菌活性及其对白色念珠菌毒力因子的抑制作用,以及生物膜的形成和清除。

研究设计/方法:采用微量稀释法、生物自显影法和时间杀伤试验,在光照和黑暗条件下,评估植物不同部位的 Hex、DCM、EtOAc 和 MeOH 提取物的抗真菌光动力活性。评估了最活跃的提取物抑制白色念珠菌毒力因子(上皮细胞粘附、芽管和假菌丝形成以及水解酶分泌)的能力。此外,还通过与 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐(MTT)反应研究了对生物膜形成和清除的活性,MTT 定量了这些结构中的活细胞。

结果

在 UVA 光照射下,来自 Thymophylla pentachaeta 根的 Hex 和 DCM 提取物对白色念珠菌表现出高的光动力抗真菌活性[最小抑菌浓度(MFC)= 7.8 µg/ml]。活性提取物(Hex 和 DCM 来自根)的化学分析表明存在光活性噻吩。这两种提取物均通过 I 型和 II 型机制产生活性氧。在光照条件下,这些提取物在亚抑制浓度下降低了白色念珠菌对颊上皮细胞(BEC)的粘附,抑制了芽管形成并减少了酯酶的产生。最后,它们对经照射的预形成生物膜表现出活性(Hex 和 DCM 提取物的 MFC 值分别为 3.91 µg/ml 和 15.63 µg/ml)。

结论

综上所述,结果表明,在 UVA 照射下,Thymophylla pentachaeta 根提取物具有很强的光动力效应,使其成为对抗白色念珠菌的现有抗真菌药物的有价值替代品。

相似文献

2
Photodynamic activity of Tagetes minuta extracts against superficial fungal infections.
Med Mycol. 2020 Aug 1;58(6):797-809. doi: 10.1093/mmy/myz114.
3
Mechanistic studies of Candida albicans photodynamic inactivation with Porophyllum obscurum hexanic extract and its isolated thiophenic compounds.
Photodiagnosis Photodyn Ther. 2019 Jun;26:420-429. doi: 10.1016/j.pdpdt.2019.05.003. Epub 2019 May 10.
7
Phytolacca tetramera berries extracts and its main constituents as potentiators of antifungal drugs against Candida spp.
Phytomedicine. 2024 Jul 25;130:155569. doi: 10.1016/j.phymed.2024.155569. Epub 2024 Mar 30.
8
Virulence factors of fluconazole-susceptible and fluconazole-resistant Candida albicans after antimicrobial photodynamic therapy.
Lasers Med Sci. 2017 May;32(4):815-826. doi: 10.1007/s10103-017-2177-y. Epub 2017 Mar 9.
9
Do raspberry extracts and fractions have antifungal or anti-adherent potential against Candida spp.?
Int J Antimicrob Agents. 2018 Dec;52(6):947-953. doi: 10.1016/j.ijantimicag.2018.08.020. Epub 2018 Sep 1.
10
Biofilm inhibition by Cymbopogon citratus and Syzygium aromaticum essential oils in the strains of Candida albicans.
J Ethnopharmacol. 2012 Mar 27;140(2):416-23. doi: 10.1016/j.jep.2012.01.045. Epub 2012 Feb 2.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验